Aged T cell subsets are characterized by a distinct microRNA signature

Abstract

Event Abstract Back to Event Aged T cell subsets are characterized by a distinct microRNA signature Nato Teteloshvili1*, Anke Van Den Berg1, Niels Van Der Geest2, Roelof Jan Van Der Lei1, Pytrick Jellema1, Elisabeth Brouwer2, Annemieke M. H. Boots2 and Bart Jan Kroesen1 1 University Medical Center Groningen, University of Groningen, Department of Pathology and Medical Biology, Netherlands 2 University Medical Center Groningen, University of Groningen, Department of Rheumatology and Clinical Immunology, Netherlands Objective Decline of immunological responsiveness in elderly correlates with changes in the composition of the T cell compartment. Recently, microRNAs (miRNA) have emerged as important players in the regulation of T cell development, differentiation and function. However, comprehensive insight into the contribution of miRNAs to T cell age-related changes remains elusive. The aim of this study was to investigate the age associated changes in miRNA expression within defined T cell subsets in young and old healthy individuals. Materials and Methods T cell subsets (naïve, memory, CD4 and CD8 cells) derived from young and elderly healthy subjects were sorted based on CD3, CD4, CD45RO and CCR7 expression. MiRNA expression patterns were determined using the agilent human miRNA microarray platform (V2) based on Sanger miRbase (release 10.1). Results were validated by miRNA-specific qRT-PCR. Results Hierarchical clustering showed differential expression of miRNAs mainly between naive and memory T-cell subsets. Age related differential expression was observed predominantly within the naive CD45RO- T-cell population. 17 miRNAs showed at least 2 fold up- or downregulation in aged naïve T cells. Analysis of individual samples revealed a statistically significant age related upregulation for miR-21, miR-223 and miR-451 by qRT-PCR. Moreover, age-related upregulation of miR-21 was associated with age-dependent accumulation of end-stage differentiated CD8 T cells. Conclusion Age-related changes in miRNA expression are found predominantly within the CD45RO- T cell compartment that converges with the accumulation of end-stage differentiated CD45RO- T cells. Keywords: T cells, Ageing, microRNA, microRNA array, microRNA expression changes Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Teteloshvili N, Van Den Berg A, Van Der Geest N, Van Der Lei R, Jellema P, Brouwer E, Boots AH and Kroesen B (2013). Aged T cell subsets are characterized by a distinct microRNA signature. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00994 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Nato Teteloshvili, University Medical Center Groningen, University of Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands, n.teteloshvili@umcg.nl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nato Teteloshvili Anke Van Den Berg Niels Van Der Geest Roelof Jan Van Der Lei Pytrick Jellema Elisabeth Brouwer Annemieke M. H Boots Bart Jan Kroesen Google Nato Teteloshvili Anke Van Den Berg Niels Van Der Geest Roelof Jan Van Der Lei Pytrick Jellema Elisabeth Brouwer Annemieke M. H Boots Bart Jan Kroesen Google Scholar Nato Teteloshvili Anke Van Den Berg Niels Van Der Geest Roelof Jan Van Der Lei Pytrick Jellema Elisabeth Brouwer Annemieke M. H Boots Bart Jan Kroesen PubMed Nato Teteloshvili Anke Van Den Berg Niels Van Der Geest Roelof Jan Van Der Lei Pytrick Jellema Elisabeth Brouwer Annemieke M. H Boots Bart Jan Kroesen Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.