Abstract
Rat serum lipoproteins were separated into at least four fractions by agarose-starch gel electrophoresis. The system used was discontinuous in that glycine and sodium barbitone buffer was used in the reservoirs and Tris buffer was used for the gels. The four major bands could be related to the pattern obtained by ultracentrifugation. The high density lipoproteins consisted of at least two poorly resolved bands and were not separated from albumin. The vertical gel apparatus was further modified to accept 0.4 ml of rat plasma, which was prestained with Sudan black. After electrophoresis the different lipoprotein bands could conveniently be cut out and the lipid phosphorus determined. The addition of Sudan black B decreased the recovery of the low and high density lipoproteins by 5-9%. However, the recovery of phospholipids was reproducible (80 +/- 2%) and the high density lipoproteins contained over two-thirds of the plasma lipid phosphorus.
Highlights
The vertical gel apparatus was further modified to accept 0.4 ml of rat plasma, which was prestained with Sudan black
The lipoprotein classes separated by ultracentrifugation can be correlated readily with the classes obtained by agarose-starch gel electrophoresis (Fig. 1)
Its heterogeneous pattern is most readily recognized during the preparative procedure using plasma prestained with Sudan black (Fig. 2)
Summary
The vertical gel apparatus was further modified to accept 0.4 ml of rat plasma, which was prestained with Sudan black. R A T SERUM or plasma lipoproteins have been separated by electrophoresis in several media, including acrylamide gel [1] and agarose gel [2, 3]. T h e slide frames of the vertical gel electrophoresis apparatusare filled with the molten agarose and the sample molds are introduced.
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