Abstract
A magnetic solid-phase extraction method based on agarose coated magnetic nanoparticles (ACMNPs) coupled to a magnetic field agitation (MFA) device was developed and used for the preconcentration and graphite furnace atomic absorption spectrometric determination of trace amounts of MoVI in beans. The formation of the nanoparticles and their encapsulation in agarose micro-flakes was conducted in a single step. The nanomagnetic agarose particles were activated by an epichlorohydrin method, functionalized by phenylephrine and used for selective preconcentration of MoVI. The influence of different analytical parameters such as pH, extraction time, type and volume of eluent and amount of adsorbent on the preconcentration of MoVI were investigated. No important interferences were observed for the determination of the analyte in presence of several other metal ions. The capacity of the adsorbent for MoVI at pH 4.0, was 13.2 µg per mL of its packed volume. Eight replicated analyses at the optimized conditions resulted in a recovery of 95.3% with a relative standard deviation of 4.9%. The detection limit of the method (3σ) for MoVI was 49 ng L-1. The method was successfully applied to the determination of MoVI in beans samples.
Highlights
Molybdenum is an essential trace element for both animals and plants
Appearance of two clear isobestic points indicates the formation of a complex between the metal ion and the ligand
In order to avoid grinding of the agarose micro-flakes during stirring by magnetic bars, the home-made magnetic field agitation (MFA) system was used for efficient agitation of the magnetic agarose particles during the adsorption/ desorption procedures.[18]
Summary
Molybdenum is an essential trace element for both animals and plants. In plants, this element is necessary for the fixation of atmospheric nitrogen by bacteria to begin the protein synthesis. The agarose coated magnetic nanoparticles (ACMNPs) are functionalized by phenylephrine (PhP), a known medicine, to be coupled to a magnetic field agitation (MFA) system and used for preconcentration of molybdenum in some beans, before determination by GFAAS.
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