African Buffalo and Colonial Cattle: Is ‘Systems Change’ the Best Future for Farming and Nature in Africa?

The African buffalo (Syncerus caffer) has been implicated as the reservoir of several bovine infectious agents. However, there is insufficient information on the protective immune responses in the African buffalo, particularly in infected animals. In this study, we analysed Th1 cytokines IL-2 and IFN-γ, and Th2 cytokines IL-4 and IL-10. The cloned cDNA of IL-2, IL-4, IL-10 and IFN-γ contained an open reading frame of 468, 501, 408 and 540 nucleotides, encoding polypeptides of 155, 166, 135 and 179 amino acids, respectively. Nucleotide sequence homology of IL-2, IFN-γ and IL-4 was more than 98% between the African buffalo and cattle, which resulted in identical polypeptides. Meanwhile, IL-10 gene of African buffalo and cattle had 95% homology in nucleotide sequence, corresponding to thirteen amino acid residues substitution. Cysteine residues and potential glycosylation sites were conserved within the family Bovinae. Phylogenetic analyses including cytokines of the African buffalo placed them within a cluster comprised mainly of species belonging to the order Artiodactyla, including cattle, water buffalo, sheep, goat, pig and artiodactyl wildlife. A deeper understanding of the structure of these cytokines will shed light on their protective role in the disease-resistant African buffalo in comparison with other closely related species.

Bovine herpesvirus 4 (BoHV-4) has been isolated from cattle throughout the world, but virological and serological studies have suggested that the African buffalo is also a natural host for this virus. It has previously been found that the Bo17 gene of BoHV-4 was acquired from an ancestor of the African buffalo, probably around 1.5 million years ago. Analysis of the variation of the Bo17 gene sequence among BoHV-4 strains suggested a relatively ancient transmission of BoHV-4 from the buffalo to the Bos primigenius lineage, followed by a host-dependent split between zebu and taurine BoHV-4 strains. In the present study, the evolutionary history of BoHV-4 was investigated by analysis of five gene sequences from each of nine strains representative of the viral species: three isolated from African buffalo in Kenya and six from cattle from Europe, North America and India. No two gene sequences had the same evolutionary tree, indicating that recombination has occurred between divergent lineages; six recombination events were delineated for these sequences. Nevertheless, exchange has been infrequent enough that a clonal evolutionary history of the strains could be discerned, upon which the recombination events were superimposed. The dates of divergence among BoHV-4 lineages were estimated from synonymous nucleotide-substitution rates. The inferred evolutionary history suggests that African buffalo were the original natural reservoir of BoHV-4 and that there have been at least three independent transmissions from buffalo to cattle, probably via intermediate hosts and--at least in the case of North American strains--within the last 500 years.

Histo-morphology of the Uterus and Early Placenta of the African Buffalo ( Syncerus caffer) and Comparative Placentome Morphology of the African Buffalo and Cattle ( Bos taurus)

African buffalo (Syncerus caffer) have been distinct from the Auroch lineage leading to domestic cattle for 5 million years, and are reservoirs of multiple pathogens, that affect introduced domestic cattle. To date, there has been no analysis of the class I MHC locus in African buffalo. We present the first data on African buffalo class I MHC, which demonstrates that gene and predicted protein coding sequences are approximately 86–87% similar to that of African domestic cattle in the peptide binding region. The study also shows concordance in the distribution of codons with elevated posterior probabilities of positive selection in the buffalo class I MHC and known antigen binding sites in cattle. Overall, the diversity in buffalo class I sequences appears greater than that in cattle, perhaps related to a more complex pathogen challenge environment in Africa. However, application of NetMHCpan suggested broad clustering of peptide binding specificities between buffalo and cattle. Furthermore, in the case of at least 20 alleles, critical peptide-binding residues appear to be conserved with those of cattle, including at secondary anchor residues. Alleles with six different length transmembrane regions were detected. This preliminary analysis suggests that like cattle, but unlike most other mammals, African buffalo appears to exhibit configuration (haplotype) variation in which the loci are expressed in distinct combinations.

Es bestehen Unterschiede in der Reproduktionsphysiologie von Afrikanischem Büffel (Syncerus caffer), Hausrind (Bos taurus) und Wasserbüffel (Bubalus bubalis). Ziel der vorliegenden Studie war ein histomorphologischer Vergleich des Baues der Gebärmutter vom güsten und trächtigen Afrikanischen Büffel mit der des Hausrindes. Zwei Uteri von güsten Büffeln wurden untersucht und das frühe Stadium der Plazentation an je sechs Präparaten von S. caffer und B. taurus verglichen (fetale Scheitel-Steiss-Längen ((fSSL)) von 2 bis 17.5 cm). Das Endometrium des Büffels besitzt runde bis ovale, kuppelförmige und drüsenfreie Karunkeln. Ein meist einfaches, hochprismatisches Epithel nicht Zilien tragender Zellen bedeckt die Karunkeln, während zusätzliche Zilien tragende Zellen über den interkarunkulären Bereichen und im zum Teil mehrreihigen Epithel der Uterindrüsen vorkommen. Während der Frühgravidität bilden sich zahlreiche Plazentome. Im Gegensatz zum Hausrind sind diese jedoch beim Büffel - bei gleicher fSSL - ohne Stiel. Die ungestielten, kuppelförmigen Plazentome des Büffels enthalten einfache und leicht konische fetale Zotten, welche sich weniger stark als beim Hausrind verzweigen. Dies lässt auf eine vergleichsweise einfachere fetomaternale Interdigitation beim Büffel schliessen. Eine synepitheliochoriale Plazentaschranke kann auch beim Büffels vermutet werden, da sowohl das Auftreten von Trophoblast-Riesenzellen als auch deren Ultrastruktur den beim Hausrind beschriebenen Verhältnissen ähneln.
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\nDifferences exist in reproductive physiology between African buffalo (Syncerus caffer), cattle (Bos taurus) and water buffalo (Bubalus bubalis). The aim of this study was to histo-morphologically compare the anatomy of non-pregnant and pregnant uteri of buffalo and cattle. Two non-pregnant uteri and placentae of six pregnant African buffalo were used. Early placentome formation (fetal crown rump length (CRL): 2-17.5 cm) in Syncerus caffer and Bos taurus was compared. The endometrium of buffalo uteri comprises round to ovoid, dome-shaped and gland-free caruncles. A predominantly simple columnar epithelium of non-ciliated cells covers caruncular tissue, while, additionally, ciliated cells occur over intercaruncular areas and within the epithelium of the endometrial glands. During early gestation, multiple placentomes develop. Unlike cattle placentomes at similar CRL, buffalo placentomes do not develop a caruncular stalk The sessile, dome-shaped buffalo placentome has simple, slightly conical villi branching less than in cattle, thus indicating a less complex feto-maternal interdigitation than seen in the latter. A synepitheliochorial interhaemal barrier can be expected in the buffalo placenta, as the occurrence and ultrastructure of trophoblast giant cells resemble those described in cattle.

Wildlife is a maintenance host for several significant livestock diseases. Interspecific pathogen transmission may occur in complex socio‐ecological systems at wild‐domestic interfaces that have so far been seldom studied. We investigated the relationship between the dynamics of foot and mouth disease (FMD) in vaccinated and unvaccinated cattle populations with respect to frequency of contacts with African buffalo at different buffalo‐cattle interfaces.A total of 36 GPS collars were deployed on African buffalo (Syncerus caffer) and cattle (Bos taurus,Bos indicus) to assess contact patterns at the periphery of 3 protected areas in Zimbabwe. Simultaneously, a longitudinal survey of 300 cattle with five repeated sampling sessions on known individuals during 16 months was undertaken. Immunological assays (ELISAs), that allowed tracking the production of antibodies following infection or vaccination, were used to assess serological transitions (i.e., incidence and reversion) in the surveyed cattle. Variation in rates of serological transitions across seasons, sites and as a function of the frequency of contact with buffalo was analyzed using generalized linear mixed models.The incidence in the cattle populations of FMD antibodies produced following infection varied among sites and as a function of contact rates with African buffalo. The incidence was higher for sites with higher contact rates between the two species. The serological incidence was also related to seasons, being higher during the dry or rainy seasons depending on sites. The reversion rate pattern was the opposite of this incidence rate pattern. Vaccination seemed partly efficient at the individual level, but it did not prevent the diffusion of FMD viruses from the wild reservoir host to the domestic cattle population. Furthermore, antibodies were detected in areas where cattle had not been vaccinated, suggesting that the virus may have spread without being detected in domestic populations.Access to resources shared by buffalo and livestock, particularly water and grazing areas during the dry season, could partly explain the observed patterns of FMD transmission. We discuss how insights on ecological processes leading to wildlife‐livestock contacts may provide some innovative solutions to improve FMD management, including surveillance, prevention or control of buffalo‐borne outbreaks, by adopting strategies targeting risky areas and periods.

African studies in the Soviet Union have deep roots in the past. The nature of Africa, the African peoples' way of life, their culture, arts, and crafts have long been of special interest to scholars in the Soviet Union. We have never had any mercenary motives, for our country never had colonies in Africa and never aimed at seizing African lands. No Russian soldier has ever been to Africa. Moreover, many Russian progressive intellectuals strongly protested against any form of exploitation and slavery. More than once they spoke in support of Africans and attacked the slave trade and the policy of turning the vast regions of Africa into what Karl Marx called ‘field reserves’ for the hunting of Africans.

BackgroundUnderstanding the epidemiology of foot-and-mouth disease (FMD), including roles played by different hosts, is essential for improving disease control. The African buffalo (Syncerus caffer) is a reservoir for the SAT serotypes of FMD virus (FMDV). Large buffalo populations commonly intermingle with livestock in Kenya, yet earlier studies have focused on FMD in the domestic livestock, hence the contribution of buffalo to disease in livestock is largely unknown. This study analysed 47 epithelia collected from FMD outbreaks in Kenyan cattle between 2008 and 2012, and 102 probang and serum samples collected from buffalo in three different Kenyan ecosystems; Maasai-Mara (MME) (n = 40), Tsavo (TSE) (n = 33), and Meru (ME) (n = 29).ResultsAntibodies against FMDV non-structural proteins were found in 65 of 102 (64%) sera from buffalo with 44/102 and 53/102 also having neutralising antibodies directed against FMDV SAT 1 and SAT 2, respectively. FMDV RNA was detected in 42% of the buffalo probang samples by RT-qPCR (Cycle Threshold (Ct) ≤32). Two buffalo probang samples were positive by VI and were identified as FMDV SAT 1 and SAT 2 by Ag-ELISA, while the latter assay detected serotypes O (1), A (20), SAT 1 (7) and SAT 2 (19) in the 47 cattle epithelia. VP1 coding sequences were generated for two buffalo and 21 cattle samples. Phylogenetic analyses revealed SAT 1 and SAT 2 virus lineages within buffalo that were distinct from those detected in cattle.ConclusionsWe found that FMDV serotypes O, A, SAT 1 and SAT 2 were circulating among cattle in Kenya and cause disease, but only SAT 1 and SAT 2 viruses were successfully isolated from clinically normal buffalo. The buffalo isolates were genetically distinct from isolates obtained from cattle. Control efforts should focus primarily on reducing FMDV circulation among livestock and limiting interaction with buffalo. Comprehensive studies incorporating additional buffalo viruses are recommended.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0333-9) contains supplementary material, which is available to authorized users.

Infection of African buffalo (Syncerus caffer) and cattle with Theileria parva lawrencei after serial passage in cattle

Mycobacterium fortuitum and at least 1 unidentified species of soil mycobacteria were isolated from lymph nodes from 4 of 5 African buffalo (Syncerus caffer) that had been culled because of positive test results using the Bovigam assay. The buffalo were part of a group of 16 free-ranging buffalo captured in the far north of the Kruger National Park (South Africa) assumed to be free of bovine tuberculosis. No Mycobacterium bovis was isolated. To investigate the possible cause of the apparent false-positive diagnosis, the Mycobacterium isolates were inoculated into 4 experimental cattle and their immune responses monitored over a 13-week period, using the gamma interferon assay. The immune reactivity was predominantly directed toward avian tuberculin purified protein derivative (PPD) and lasted for approximately 8 weeks. During that period 3 of 4 cattle yielded positive test results on 1 or 2 occasions. The immune responsiveness was boosted when the inoculations were repeated after 15 weeks, which led to 2 subsequent positive reactions in the experimental animal that did not react previously. Including an additional stimulatory antigen, sensitin prepared from M. fortuitum in the gamma interferon assay, showed that it was able to elicit a detectable gamma interferon response in all 4 experimentally inoculated cattle when applied in parallel with bovine and avian tuberculin PPD for the stimulation of blood samples. The implications of occasional cross-reactive responses in natural cases of infection with environmental mycobacteria in the diagnosis of bovine tuberculosis in African buffalo and cattle in South Africa are discussed.

Foot-and-mouth disease virus (FMDV) serotype SAT3 is a rarely studied serotype primarily circulating in southern Africa, with African buffalo (Syncerus caffer) serving as its key reservoir. In this study, we performed a comprehensive phylogenetic and phylodynamic analysis of SAT3 based on 81 full-length VP1 gene sequences collected between 1934 and 2018. Maximum likelihood and Bayesian analyses revealed five distinct topotypes, each with clear geographic and host associations. Notably, topotypes I, II and III were observed in both African buffalo and cattle (Bos taurus), while topotype IV appeared restricted to African buffalo. Likelihood mapping indicated moderate to strong phylogenetic signal, and the mean substitution rate was estimated at 3.709 × 10−3 substitutions/site/year under a relaxed molecular clock. The time to the most recent common ancestor (TMRCA) was traced back to 1875. Discrete phylogeographic reconstruction identified Zimbabwe as a major center, with multiple supported cross-border transmission routes. Host transition analysis further confirmed strong directional flow from buffalo to cattle (BF = 1631.09, pp = 1.0), highlighting the wildlife–livestock interface as a key driver of SAT3 persistence. Together, these results underscore the evolutionary complexity of SAT3 and the importance of integrating molecular epidemiology, spatial modeling, and host ecology to inform FMD control strategies in endemic regions.

As World War II came to a close, there was neither any national park legislation nor any national department in British East and Central Africa (comprising Anglo-Egyptian Sudan, Uganda, Kenya, Tanganyika, Northern and Southern Rhodesia, and Nyasaland). Not all of colonial territories in region even had a game department. The existing departments were minute in relation to size of their territories and none possessed any sort of research agenda or scientifically trained staff member. Fifteen years later, situation had shifted 18o degrees. Colonial development funds poured into improvement of new national across region, airplanes crisscrossed skies overhead to count wildlife herds, and scores of biologists and ecologists moved over landscape to analyze habits and histories of parks faunal residents. The shift represented a veritable conservation boom and dozens of new appeared as bright green patches on previously monochrome maps. What had happened in immediate postwar years to change status of national and wildlife conservation so dramatically? Some historians have traced origins of twentieth-century wildlife conservation to psychological and emotional importance of African environment for European colonizers. Accordingly roots of British colonial national park and wildlife laws have been traced to culturally constructed ideas of nature in Africa. Historians have offered concepts such as the Eden complex or the cult of Hunt' to explain European, particularly British, fascination with African wildlife and anxieties over its disappearance. The core of their argument is that idea of Africa as symbolic Eden has stimulated western interests in African conservation through colonial period and into present. Without dismissing their importance in shaping British attitudes toward nature in Africa, elite European cultural values do not fully explain explosion in conservation initiatives in final years of colonialism. There had been many proposals for national in first half of century and an international wildlife treaty as early as 1900, but territorial governments in Africa largely ignored these until 1940s.

Tick-borne diseases caused by Theileria are of economic importance in domestic and wildlife ruminants. The majority of Theileria infects a limited number of host species, supporting the concept of host specificity. However, some Theileria seem to be generalists challenging the host specificity paradigm, such as Theileria sp. (sable) reported from various vertebrate hosts, including African buffalo, cattle, dogs and different antelope species. We tested the hypothesis that T. sp. (sable) uses Bovidae as hosts in general using a real-time polymerase chain reaction assay specific for T. sp. (sable) and a closely related genotype: T. sp. (sable-like). Various antelope species from the Tragelaphini (black wildebeest, blesbuck, blue wildebeest, gemsbuck, sable and waterbuck) tested positive for either T. sp. (sable) or T. sp. (sable-like). However, no African buffalo (n = 238) or cattle (n = 428) sampled in the current study tested positive, suggesting that these latter species are not carrier hosts. The results were confirmed using next-generation sequencing which also indicated at least 13 new genotypes or species found in various antelope and giraffes. Genotypes were found in single host species or in evolutionarily related hosts, suggesting that host specificity in Theileria may be a lineage specific phenomenon likely associated with tick-host-parasite co-evolution.

Teneral Glossina morsitans centralis were fed on the flanks of African buffalo, N'Dama or Boran cattle infected with Trypanosoma vivax IL 2337. The infected tsetse were maintained on goats and on day 25 after the infected feed, the surviving tsetse were dissected to determine the infection rates. The mean mature infection rates (% +/- S.E.) in the tsetse fed on buffalo, N'Dama and Boran cattle were 34.3 +/- 9.9, 33.7 +/- 13.4 and 58.9 +/- 7.1, respectively. Logistic regression analysis indicated that infection rates in the labrum and hypopharynx of the tsetse were significantly lower when fed on the infected buffalo or N'Dama than Boran cattle. Similarly, the risk of infection was significantly lower in male than female tsetse. When teneral G. m. centralis, G. pallidipes, G. p. gambiensis, G. brevipalpis and G. longipennis were fed simultaneously on either the buffalo cow, the N'Dama bull or the Boran steer infected with T. vivax IL 2337, the mature infection rates were higher in the two morsitans group than the two fusca group tsetse, whilst G. p. gambiensis was relatively refractory to the infection, irrespective of the host species on which they fed. Logistic regression analysis indicated that the infection rates in the labrum and hypopharynx were significantly different amongst the five tsetse species for each of the three infected host animals. Nevertheless, the trypanotolerant African buffalo and N'Dama cattle may serve as reservoirs of T. vivax infection as can trypanosusceptible Boran cattle.

This study investigated the foraging behaviours, host species utilisation, and spatial attachment preferences of Red‐billed Oxpecker ( Buphagus erythrorhynchus ) and Yellow‐billed Oxpecker ( Buphagus africanus ) in Salambala Conservancy, northeastern Namibia, from May to September 2023. A total of 1252 potential host animals were observed, including African buffalo ( Syncerus caffer ), cattle ( Bos taurus ), giraffe ( Giraffa camelopardalis ), and plains zebra ( Equus quagga ). Oxpecker‐host interactions were recorded for 234 Red‐billed Oxpecker and 69 Yellow‐billed Oxpecker. Red‐billed Oxpecker primarily exhibited perching and tick‐feeding behaviours, particularly on buffalo and cattle, while Yellow‐billed Oxpecker showed a stronger reliance on tick‐feeding, especially on buffalo. Other feeding behaviours, such as the consumption of ear wax, mucus, and saliva, were mainly recorded on giraffe, suggesting resource diversification strategies. Host rejection and tolerance behaviours also differed among species, with buffalo and giraffe demonstrating higher tolerance levels compared to cattle and zebra, which exhibited elevated rejection responses. Oxpecker attachment patterns differed across host species and body regions. Preferred foraging regions included the back, head, and anogenital areas that are typically difficult for hosts to self‐groom. These findings provide dry‐season baseline data on oxpecker‐host interactions in a mixed‐use conservancy landscape and highlight behavioural flexibility in response to host species and tolerance. Interpretation of interspecific differences, particularly for Yellow‐billed Oxpeckers, should be viewed as preliminary due to uneven sampling across hosts.