Abstract
The triazine dye C.I. Reactive Blue 2 has been immobilised on a particulate perfluorocarbon support by preparation of a hydrophilic polymeric coating comprising polyvinyl alcohol (average molecular weight, M r 14 000) esterified with perfluorooctanolyl chloride and securely adsorbed ont he perfluorocarbon support by multiple Van der Waals interactions. This polyvinyl alcohol-based coating wets the perfluorocarbon support and provides a neutral barrier to non-specific adsorption of proteins. Reaction with the triazine dye C.I. Reactive Blue 2 allows secure immobilisation of this versatile pseudo-affinity ligand and yields a remarkably stable adsorbent. The performance and capacity of the perfluorocarbon-based adsorbent was assessed: an 8-fold purification of lactable dehydrogenase was achieved from a crude rabbit muscle extract in 100% yield. The albumin-binding capacity of a dyed perfluorocarbon support containing 2.2±0.2 μmol dye per g wet support was determined to be 15.7 mg/ml by frontal analysis. These supports compare favourably with conventional polysaccharidic affinity supports.
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