Abstract
Affinity chromatography operated in a tapered-bed with the continuous up-flow was researched. Purification of Lysozyme by Blue Dextran Sepharose 4B was chosen as a model system. A series of experiments such as the batchwise adsorption, tapered columnwise adsorption, elution and pressure drop determination were carried out to find out characteristics such as adsorption equilibrium, hydrodynamic performance and adsorption efficiency for this kind of new affinity process. A mathematical model to describe this system was also established and verified. Research results showed that the affinity purification by the tapered-bed with up-flow could be operated in three regimes, i.e. the fixed-bed regime, fully fluidized-bed regime and partially fluidized-bed regime, which depended on the flow rate. The adsorption efficiencies were the highest in the fixed-bed regime, and poor adsorption efficiencies were shown in the fully fluidized-bed regime.
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