Adventitious shoot regeneration of pear (Pyrus spp.) genotypes

Abstract

Adventitious shoot regeneration of twenty-four pear genotypes was compared in a common in vitro shoot induction and development protocol. This study also compared cultures newly established from scionwood with cultures that been in long-term cold storage. In vitro cultures of 13 Pyrus genotypes and budwood from 23 Pyrus genotypes were obtained from the National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon. With the exception of one genotype of P. elaeagrifolia Pall., and ‘Ya Li’ (P. pyrifolia var. sinensis Teng & Tanabe), all were P. communis L. cultivars. The basal shoot induction media consisted of Chevreau and Leblay (CL) basal nutrients, vitamins, and organics (Chevreau and Leblay in Acta Hortic 336: 263–268, 1993). The analysis of variance indicated that differences among genotypes were highly significant and the main effect of culture origin was non-significant. However, there was a significant interaction between genotype and culture origin, with percentage regeneration of ‘Abate Fetel’ from new budwood significantly greater than that from long-term in vitro cultures, while ‘Jesinji Vodenac’ cultures derived from the old NCGR cultures regenerated significantly more adventitious shoots. The ranges of mean regeneration frequency were similar for both in vitro (0–87.7%) and scionwood-derived cultures (0–70.7%). Maximum regeneration was observed for ‘Conference’, followed by ‘Magness’, ‘Dr. Jules Guyot’, and Packham’s Triumph’. The range of number of adventitious shoots was relatively narrow, with the minimum of 1.0 for seven genotypes to 2.2 for ‘Conference’.