Abstract
A novel in vitro protocol was developed for regeneration of adventitious cumin shoots from explants of hypocotyl and stem internodal segments. The best response towards shoot induction was obtained from explant tissues of stem internodes when placed slightly embedded (3–4 mm) in Murashige and Skoog (MS) solid medium containing 2.5 μM benzyladenine (BA). No shoot initiation was observed on medium lacking BA. The induced shoot initials on BA-containing medium, proliferated and elongated during secondary culture on the medium lacking plant growth regulators (PGR). Up to 30 shoots per explant were harvested on this medium. Although, these shoots readily formed roots (95%) on PGR-free medium, only 30% of them survived after the acclimatization to the greenhouse conditions. The plant survival rate ex vitro was enhanced (73%) when the excised shoots were cultured for rooting in the medium with 2% polyethylene glycol (PEG 6000). Adding 1 μM indole-3-butyric acid (IBA) to the PEG-containing medium increased the percentage of rooting from 75 to 85%.
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