Adventitious rooting of Chrysanthemum is stimulated by a low red:far-red ratio

Abstract

Adventitious rooting, a critical process in the vegetative propagation of many ornamentals, can be affected by both light intensity and light quality. We investigated the use of spectral light quality to improve adventitious rooting of Chrysanthemum morifolium cuttings by applying different combinations of blue, red and far-red light. Additionally, unrooted cuttings were treated before planting with two auxin transport inhibitors (TIBA and NPA) to study the effect of light quality on auxin biosynthesis and/or transport. Results showed that lowering the R:FR ratio (decreasing the phytochrome photostationary state, PSS) improved rooting significantly and decreased the inhibiting effect of the auxin transport inhibitor NPA. An extra decrease of PSS by adding blue light to a red + far-red spectrum further enhanced rooting. In contrast, adding blue light to solely red light decreased rooting, an effect which was more pronounced in combination with the auxin transport inhibitors TIBA and NPA. Our results show that phytochrome plays a role in adventitious root formation through the action of auxin, but that also blue light receptors interact in this process.