Abstract
Background Although (Elaeis guineensis) is planted on only 5% of the total world vegetable oil acreage, it accounts for more than 30% of vegetable oil produced worldwide [1]. In Brazil, the Federal Government launched a plan to boost Oil Palm production as a way to meet the biofuels market increasing demand for vegetable oil. Nevertheless, the Oil Palm expansion areas in Brazil coincide with the area of occurrence of bud rot [2], a major disease that is decimating plantations already established in the area. In a way to circumvent such problem, breeders are now using E. oleifera germplasm in Oil Palm breeding programs, generating inter-specific hybrids not only resistant to bud rot, but with higher unsaturated fatty acid content, lower height [3]. E. oleifera, however, lack the genomic resources currently available for Oil Palm [1], hampering many possible studies that could potentially help breeding. Based on the foregoing, we started a project to develop a large set of molecular markers for the species based on the DArTSeq platform [4].
Highlights
(Elaeis guineensis) is planted on only 5% of the total world vegetable oil acreage, it accounts for more than 30% of vegetable oil produced worldwide [1]
A combination of restriction enzymes (RE) was used to reduce the complexity of the samples that were later pooled sequenced in the Illuminas HiSeq 2000 platform
Based on the alignment of the reads to the reference sequence we’ve discovered 5.500 high-quality SNP loci, and other 1.950 lower-quality SNPs. This set of polymorphic markers may be used for a series of downstream applications on palm genetics and genomics
Summary
(Elaeis guineensis) is planted on only 5% of the total world vegetable oil acreage, it accounts for more than 30% of vegetable oil produced worldwide [1]. Methods We collected 552 leaf samples from 206 accessions of the Embrapa E. oleifera germplasm collection. DNA was extracted and purified and sent for DArT-Pty for DArTSeq development.
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