Abstract
The derivatization chemistry for conversion of mixtures of oligopeptides to the corresponding N-α, (ω)-trifluoroethyl-O-trimethylsilyl polyamino alcohols, the derivatives of choice for the sequencing of polypeptides by gas chromatographic mass spectrometry, has been optimized. The improvements have minimized undesirable side reactions and resulted in a five- to tenfold increase in sensitivity over previous methods employing either lithium aluminum deuteride or hexadeuterodiborane as the reducing agent. For derivatives of certain very polar peptides increases in yield have exceeded 100-fold. The procedure has been evaluated with mixtures of synthetic oligopeptides and used in the course of the determination of the amino acid sequence of the membrane protein bacteriorhodopsin, as well as other proteins.
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