Abstract
In recent years, researchers have tried to include living cells into electrospun nanofibers or droplets, leading to the field of live cell electrospinning and bio-electrospraying. In live cell electrospinning and bio-electrospraying,cells are embedded in a polymer and subject to the process of mechanical and electrical stimulation of the process. The resulting nanofiber mats or droplets with embedded cells have several potential applications in tissue engineering. The nanofiber structure provides a supportive and porous environment for cells to grow and interact with their surroundings. This can be favorable for tissue regeneration, where the goal is to create functional tissues that closely mimic the extracellular matrix. However, there are also challenges associated with live cell electrospinning and electrospraying, including maintaining cell viability and uniform cell distribution within the nanofiber mat. Additionally, the electrospinning/electrospraying process can have an impact on cell behavior, phenotype, and genotype, which must be cautiously monitored and studied. Overall, the goal of this review paper is to provide a comprehensive and critical analysis of the existing literature on cell electrospinning and bio-electrospraying.
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