Adriatic Hunters, Fishers and Herders

Storing information and circulating it between individuals and groups is a critical behaviour that signals a tipping point in our evolutionary history. Such practices enabled the preservation and consolidation of knowledge over extended periods, facilitating the accumulation of cultural innovations across generations. In this study, we used Social Network Analysis methods to explore how knowledge circulated during the transition to agriculture in the Western Mediterranean region. Previous studies have shown that specific elements of the material culture reveal distinct patterns of cultural interaction among early farming communities. Here, we investigated if two archaeological proxies, personal ornaments and pottery decorations, both with an exclusively symbolic function, reveal different network structures, and if the different degree of connexions acted equally on the transmission of styles, symbols, and network changes over time. Our results relied on cultural data recorded from 77 archaeological occupations covering Italy, France, and Spain, spanning over 1,500 years (ca. 7950~6450 cal BP). By utilizing a chronological dataset comprising 114 radiocarbon dates, we revealed that pottery decorative techniques networks exhibited stronger connexions over space and time, with nodes organized in clear cluster, when compared to personal ornaments networks. The findings highlight the regionalization and fragmentation of cultural networks during the Early Neolithic, and that the transmission of cultural traits within each category of artefact operated through varying cultural and social mechanisms. Pottery expressed a dynamic regional identity, continuously shaped by geographical and chronological proximity, while bead-type associations contributed to enduring identities shared across vast geographical scales. These networks shed light on the multifaceted shaping of social and symbolic capital among the Mediterranean’s early farmers, emphasizing the strength and quality of social ties that existed between communities and the level of reciprocity and cooperation required to foster these diverse social, economic, and cultural development strategies.

Storing information and circulating it between individuals and groups is a critical behaviour that signals a tipping point in our evolutionary history. Such practices enabled the preservation and consolidation of knowledge over extended periods, facilitating the accumulation of cultural innovations across generations. In this study, we used Social Network Analysis methods to explore how knowledge circulated during the transition to agriculture in the Western Mediterranean region. Previous studies have shown that specific elements of the material culture reveal distinct patterns of cultural interaction among early farming communities. Here, we investigated if two archaeological proxies, personal ornaments and pottery decorations, both with an exclusively symbolic function, reveal different network structures, and if the different degree of connexions acted equally on the transmission of styles, symbols, and network changes over time. Our results relied on cultural data recorded from 77 archaeological occupations covering Italy, France, and Spain, spanning over 1,500 years (ca. 7950~6450 cal BP). By utilizing a chronological dataset comprising 114 radiocarbon dates, we revealed that pottery decorative techniques networks exhibited stronger connexions over space and time, with nodes organized in clear cluster, when compared to personal ornaments networks. The findings highlight the regionalization and fragmentation of cultural networks during the Early Neolithic, and that the transmission of cultural traits within each category of artefact operated through varying cultural and social mechanisms. Pottery expressed a dynamic regional identity, continuously shaped by geographical and chronological proximity, while bead-type associations contributed to enduring identities shared across vast geographical scales. These networks shed light on the multifaceted shaping of social and symbolic capital among the Mediterranean's early farmers, emphasizing the strength and quality of social ties that existed between communities and the level of reciprocity and cooperation required to foster these diverse social, economic, and cultural development strategies.

In order to review evidence of human-animal relations, the paper offers an overview of the customs and funerary traditions of the Square Mouthed Pottery culture, between c. 5000 and 4300 calBC. We focus on the importance of domestic and wild animals on the basis of an analysis of grave-goods, funerary rites and personal ornaments. We also consider recent discoveries of peculiar offerings of animals and some dog burials. The evidence testifies to a diffusion of a wild component, symbolically emphasising the importance of the hunter identity in a society where subsistence actually depends primarily on domestic animals. Therefore, a contrast is drawn between the everyday and the symbolic worlds.

The rapid diffusion of farming technologies in the western Mediterranean raises questions about the mechanisms that drove the development of intensive contact networks and circulation routes between incoming Neolithic communities. Using a statistical method to analyze a brand-new set of cultural and chronological data, we document the large-scale processes that led to variations between Mediterranean archaeological cultures, and micro-scale processes responsible for the transmission of cultural practices within farming communities. The analysis of two symbolic productions, pottery decorations and personal ornaments, shed light on the complex interactions developed by Early Neolithic farmers in the western Mediterranean area. Pottery decoration diversity correlates with local processes of circulation and exchange, resulting in the emergence and the persistence of stylistic and symbolic boundaries between groups, while personal ornaments reflect extensive networks and the high level of mobility of Early Neolithic farmers. The two symbolic productions express different degrees of cultural interaction that may have facilitated the successful and rapid expansion of early farming societies in the western Mediterranean.

Molluscs emergent, Part II: themes and trends in the scientific investigation of molluscs and their shells as past human resources

The Neolithic transition from hunting and gathering to farming and cattle breeding marks one of the most drastic cultural changes in European prehistory. Short stretches of ancient mitochondrial DNA (mtDNA) from skeletons of pre-Neolithic hunter-gatherers as well as early Neolithic farmers support the demic diffusion model where a migration of early farmers from the Near East and a replacement of pre-Neolithic hunter-gatherers are largely responsible for cultural innovation and changes in subsistence strategies during the Neolithic revolution in Europe. In order to test if a signal of population expansion is still present in modern European mitochondrial DNA, we analyzed a comprehensive dataset of 1,151 complete mtDNAs from present-day Europeans. Relying upon ancient DNA data from previous investigations, we identified mtDNA haplogroups that are typical for early farmers and hunter-gatherers, namely H and U respectively. Bayesian skyline coalescence estimates were then used on subsets of complete mtDNAs from modern populations to look for signals of past population expansions. Our analyses revealed a population expansion between 15,000 and 10,000 years before present (YBP) in mtDNAs typical for hunters and gatherers, with a decline between 10,000 and 5,000 YBP. These corresponded to an analogous population increase approximately 9,000 YBP for mtDNAs typical of early farmers. The observed changes over time suggest that the spread of agriculture in Europe involved the expansion of farming populations into Europe followed by the eventual assimilation of resident hunter-gatherers. Our data show that contemporary mtDNA datasets can be used to study ancient population history if only limited ancient genetic data is available.

Personal ornaments, especially those made from the shells of marine mollusks and animal teeth, have been recovered from many Mesolithic sites across Europe. This paper reviews the evidence of personal ornaments from the Mesolithic of the Iron Gates, where such finds were identified in five sites on the Romanian bank: the cave and rock shelter sites of Climente II and Cuina Turcului, and three open-air sites—Icoana, Ostrovul Banului, and Schela Cladovei. The ornaments from these sites were made from the shells of several gastropod taxa and at least one species of dentaliid scaphopod, as well as the pharyngeal teeth of cyprinids, the teeth of several species of terrestrial mammal, fish vertebrae, and pieces of antler and bone. Particular attention is given to taxonomic identification and questions of taphonomy, provenance, selection, manufacture, and use. Experiments were conducted in which several types of ornament were replicated. Archeological and experimental pieces (at various stages of production) were examined under a microscope, to establish the durability of the beads and estimate the length of time over which they were worn. Our results show that shells of Lithoglyphus and Theodoxus sp. were simply perforated and fixed in composed adornments, as were shells of Tritia neritea in the early part of the time range. In the later Mesolithic, T. neritea shells were processed in a different way and fixed to clothing in the manner of appliques. No technological modification of the cyprinid teeth was observed; these were sewn individually onto clothing by means of a thread coated with an adhesive substance. The other categories of personal ornament were used mainly as pendants. Experimental use-wear analysis suggests that many ornaments were used over long periods, with broken or missing pieces replaced when necessary.

I applied state-of-the-art ancient DNA (aDNA) approaches to recover highly degraded DNA molecules from post-glacial hunter-gatherers and early farmers from the Mediterranean to investigate the demographic processes that underlie archaeological transitions after the Ice Age and during the expansion of farming practises. I placed a particular emphasis on examining the genomic evidence for crossings of the Mediterranean Sea, as was proposed by archaeologists based on similarities in material culture. Each of the manuscripts contributes crucial genomic reference points for Mediterranean areas where human occupation locally persisted during the Ice Age, or with some of the earliest indications for farming. In Manuscript A, I report the oldest ancient genome-wide data for Africa to date retrieved from nine ~15,000 calBP microlithic Iberomaurusian hunter-gatherers from the Mediterranean coast in Morocco (Van de Loosdrecht et al. 2018. Science). In Manuscript B, I report a genomic transect of ten individuals from the Iberian Peninsula from various sites with Upper Palaeolithic to Middle Neolithic occupations (~13,000-6,000 calBP) (Villalba-Mouco et al. 2019. Curr. Biol). In Manuscript C, I explore the multi-disciplinary potential of archaeogenetics, and jointly report genomic and isotopic data for dietary inference for a transect of 19 Mesolithic foragers and Early Neolithic farmers from Sicily (~10,700-7,200 calBP) (Van de Loosdrecht et al. 2020. BioRxiv). In the discussion I provide archaeogenetic perspectives on topics long been debated by scholars of Mediterranean prehistory: 1) the origin of the Iberomaurusian industry in Northwest Africa; 2) the admixture dynamics of European foragers after the Ice Age; 3) the genomic evidence for direct population interactions across the Mediterranean Sea; 4) the demographic processes underlying the transition from foraging to farming in the Mediterranean; and 5) the southern Mediterranean Neolithic expansion route.

A large body of information on early farming in Europe and the Near East has accumulated during the last twenty years of prehistoric research. The economies of most early farming settlements in Europe are characterised by the use of various domesticated species (wheat, barley, sheep, cattle and pig) combined with the exploitation of wild plants and animals. The view generally held sees early farming of the type practised in Europe as having originated in the Near East (Ucko & Dimbleby I969). Thence it appears to have spread first to Greece and the Balkans and subsequently over the rest of Europe (Clark 1965; Neustupny 1968). The extension of early farming across Europe is a subject of interest to a number of disciplines ranging from prehistory and palaeoecology to genetics. In this article, we concentrate on one aspect of the question: the measurement of the rate of spread or diffusion. A previous attempt to measure 'neolithic diffusion rates' was made by Edmonson (I96I). This pioneer study is unsatisfactory in a number of ways: notably in the method of measurement and in the assumption that what was being measured was a rate of cultural or stimulus diffusion.

The earliest introduction of livestock (cattle, goats, sheep, pigs) into the Carpathian Basin was an important step towards farming expansion into continental Europe. This spread beyond the environments of the southern Balkans was accompanied by a reduction in the spectrum of cultivated crops, changes in the relative representation of different domestic animals, and, most likely, adaptations of husbandry practices. How the earliest farmers in the Carpathian Basin kept their domestic stock is still understudied. We explored early animal management and land use strategies at the Starčevo settlement at Alsónyék-Bátaszék, Hungary (Early Neolithic, ca. 5800-5600 cal BC). Settled at the intersection of wide alluvial plains, waterlogged meadows and marshes to the east, and forested hills to the west, early farmers at Alsónyék had a wide variety of options for nourishing their livestock. We performed stable isotope ratio analysis of bone collagen (n = 99; δ13C, δ15N) and tooth enamel (nteeth = 28, sequentially sampled for δ13C and δ18O) from wild and domestic animals to locate them in the landscape and investigate herding practices on a seasonal scale. The bone collagen isotope ratios mostly indicate feeding in open environments. However, results from the sequential analysis of cattle and sheep enamel suggest diverse dietary strategies for winters, including consumption of forest resources, consumption of summer hay and grazing in an open environment. Most pigs appear to have had herbivorous diets, but several individuals likely supplemented their diet with animal protein. Stable isotope ratio results from the Lengyel phase at Alsónyék (ca. 4800-4300 cal BC) suggest more access to animal protein for pigs, and feeding in more open areas by wild boar, red deer and cattle compared to the Starčevo phase. This study's results demonstrate considerable variability in early animal husbandry practices at Alsónyék.

Ticks infesting wild and domestic animals and humans of Sri Lanka with new host records

Domesticated grain crops evolved from wild plants under human cultivation, losing natural dispersal mechanisms to become dependent upon humans, and showing changes in a suite of other traits, including increasing seed size. There is tendency for seed enlargement during domestication to be viewed as the result of deliberate selection for large seeds by early farmers. However, like some other domestication traits, large seeds may have evolved through natural selection from the activities of people as they gathered plants from the wild, or brought them into cultivation in anthropogenic settings. Alternatively, larger seeds could have arisen via pleiotropic effects or genetic linkage, without foresight from early farmers, and driven by selection that acted on other organs or favored larger plants. We have separated these unconscious selection effects on seed enlargement from those of deliberate selection, by comparing the wild and domesticated forms of vegetable crops. Vegetables are propagated by planting seeds, cuttings, or tubers, but harvested for their edible leaves, stems, or roots, so that seed size is not a direct determinant of yield. We find that landrace varieties of seven vegetable crops have seeds that are 20% to 2.5‐times larger than those of their closest wild relatives. These domestication effect sizes fall completely within the equivalent range of 14% to 15.2‐times for grain crops, although domestication had a significantly larger overall effect in grain than vegetable crops. Seed enlargement in vegetable crops that are propagated vegetatively must arise from natural selection for larger seeds on the occasions when plants recruit from seed and are integrated into the crop gene pool, or via a genetic link to selection for larger plants or organs. If similar mechanisms operate across all species, then unconscious selection during domestication could have exerted stronger effects on the seed size of our staple crops than previously realized.

The archaeobotanical record of 24 sites from the Neolithic period (5400–2300 cal bc) in the north-east of the Iberian Peninsula is evaluated. Remarkable amounts of data have recently been obtained for the early and middle Neolithic phases. Most of the studied sites were dry and they only yielded charred plant material. Among dry sites, several types of context were evaluated: dwelling areas, hearths, roasting pits and byres. Material was also analysed from a waterlogged cultural layer of one early Neolithic lakeshore site, La Draga. Quercus sp. (acorns), Corylus avellana L. (hazelnuts), Pistacia lentiscus L. (mastic fruits) and Vitis vinifera L. var. sylvestris (wild grapes) were among the most frequently encountered fruits and seeds. Their presence in the archaeobotanical record clearly maps their past ecological distribution in the region. There are differences observed between the charred dry-land material and the waterlogged uncharred material. Wild fruits were mostly present in an uncharred state in La Draga. Therefore, their consumption could go unnoticed in dry sites when fruits were eaten raw or without roasting. Larger amounts of charred remains of certain wild fruits like acorns and hazelnuts found in mountain areas are highlighted as potential evidence of the regular practice of roasting, potentially indicating regional traditions. All in all, our results support an intensive wild plant use at least during the first 1,300 years of the Neolithic period. Evidence of wild plant food consumption becomes scanty towards the second phase of the middle and the late Neolithic (4th and 3rd millennium cal bc). This, however, might also be due to taphonomic reasons.

This paper closely examines the process of manufacture of a largely disregarded class of social objects - personal ornaments. Specifically, the manufacture from marine mollusc shell of one distinct type of disc bead based on the archaeological remains from Franchthi Cave is described in detail, supplemented by ethnographic and experimental evidence. The results of this study establish criteria which may be applied to the production of diverse ornaments of various materials and from sites of many regions and dates. Several current models of craft specialization are compared in the following discussion. In light of these models, the evidence for the manufacture of shell beads at Franchthi Cave is re-examined and the possibility that this constituted a specialized craft production is evaluated.

Simple SummaryCrimean-Congo hemorrhagic fever is a tick-borne and zoonotic emerging viral disease that is characterized by the sudden development of high fever and vascular bleeding in humans. The seroevidence among livestock has been reported, but it is less known in wild animals. Due to the importance and emerging state of the disease, we conducted a serosurvey on both domestic and wild animals in different areas of Turkiye. Serological investigations conducted on cattle, goats, and sheep revealed 10.81%, 15.15%, and 19.23% seropositivity, respectively, in the collected serum samples. We also found seropositivity rates in hare (23.81%) and wild boars (2.5%) indicating the substantial role of wild animals in virus epidemiology in Turkiye. This study provides first seroevidence of Crimean Congo hemorrhagic fever in wild animals in Turkiye. Crimean-Congo hemorrhagic fever virus (CCHFV) is a zoonotic, tick-borne pathogen that is endemic to some parts of Europe, Africa, and Asia. The disease causes fever and hemorrhagic manifestations in humans but not in animals. Domestic and wild animals are asymptomatic hosts of CCHFV and are critical in the transmission cycle. Hyalomma marginatum spp. has been identified as the natural reservoir and vector of the virus in Turkiye. A few studies have been conducted on domesticated animals showing the seroprevalence of CCHFV in them, but seroevidence in wild animals is absent. For contributing this antrum to the understanding of virus transmission in Turkiye, we performed a seroprevalence investigation of CCHFV in both wild and domesticated animals in various geographical areas of Turkiye. In-house IgG iELISA was performed for the screening of sera IgG in a total of 582 animal samples collected from boar (n = 40), cattle (n = 259), goat (n = 132), hare (n = 21), and sheep (n = 130). Results from ELISA performed on domestic animals revealed 10.81%, 15.15%, and 19.23% anti-CCHF virus seropositivity in cattle, goats, and sheep, respectively, in collected serum samples. ELISA tests performed in wild animals showed 23.81% and 2.5% positivity in hare and wild boars, respectively, suggesting the importance of wild animals in CCHF virus epidemiology in Turkiye. This study performed the first serological investigation of CCHFV in wild animals and provided the first seroevidence of CCHFV in wild boars and hare in Turkiye.