Administration of Aspirin and Aqueous Leaf Extract of Terminalia Catappa Reduces Leukocytosis with Risk of Developing Thrombocytosis in Diabetic Wistar Rats.

Diabetes mellitus is a metabolic disorder in human and animal, particularly in pets. This study is conducted to evaluate the effect of Honey on serum levels of glucose, triglyceride, cholesterol, VLDL, LDL and HDL in experimental diabetes mellitus in rat. Male Wistar rats randomly allocated in four groups: normal, honey control, diabetic control and diabetic rats receiving honey. For creation of diabetes, a single dose of Alloxan (100 mg/kg) injected subcutaneously. After observing diabetes symptoms in groups received Alloxan, we have initiated to feed treatment group and diabetic treatment group with the equal ratio of honey and pellet. The diabetic control and control group fed only with pellet. These groups fed for 10 days. Blood samples collected from whole groups at the end of 10th day. Evaluation of the serum levels of glucose, cholesterol and LDL significantly increased between diabetic group with control group and also between treatment group and diabetic treatment group with diabetic Evaluation of the serum levels of triglyceride did not reveal statistically significant differences in diabetic treatment group and treatment group with control group, but decreased significantly compared to the diabetic control group (P<0.05). Evaluation of the serum Levels of VLDL decreased significantly in diabetic treatment group and treatment group compared with the diabetic control group (P<0.05), but did not reveal statistically difference compared with the control group. Evaluation of the serum levels of HDL did not reveal statistically significant difference between groups. Honey could be used as a natural drug for preventing diabetes mellitus disorders.

Background Research demonstrated that vitamin D3 mediated by its receptor has the potent nonclassical effects,including immunomodulatory,antiinflammatory,and neuroprotective properties,and it can enhance the secretion and sensitivity of insulin and therefore down-regulate hyperglycemia and attenuate the corneal edema.Objective The present study was to investigate the protective effect of vitamin D3on ocular structure in experimental diabetic rat. Methods Twenty-two healthy SPF C57BL/6 rats were randomly divided into vitamine D3 group (8 rabbits),diabetic control group ( 11 rabbits) and normal control group ( 3 rabbits).2％ streptozotocin ( STZ,175 mg/kg)was intraperitoneally injected to create the diabetic models in the rats of the vitamine D3 group and diabetic control group.Blood glucose was examined for 3 times in the third day after STZ injection,and the rats with the blood glucose concentration ＞16.7 mmol/L was identified as the successful diabetic models.After modeling,the rat tail blood was collected for the monitoring of blood glucose.Two weeks after modeling,vitamine D3 was intraperitoneally injected in each week for 5 times.The fundus was examined using direct ophtalmoscope,and the eyeballs were obtained under the excessive anesthesia for the measurement of thickness of the central cornea,retina and choroids by histopathological examination once a week for 7 weeks after administration of vitamin D3.The administration of the animals complied with the Statement of ARVO. Results The corneal edema appeared with the corneal thickness of (339.14± 11.13) μm in the first week and gradually attenuated with time elapse after modeling in the diabetic group ( F =382.446,P =0.000).The corneal thickness values were significantly decreased from the second week through the seventh week in the vitamin D3 group compared with diabetic control group(P＜0.05).The atrophy of the corneal epithelium was found from the fifth week to the seventh week in diabetic control group,but that in vitamin D3 group was slight (P＜0.05).The gradually thinning of the choroids was seen from the first week to the seventh week in the diabetic control group ( F =437.411,P =0.000 ),however,the thickness values in the vitamin D3 group were significantly increased in comparison with the diabetic control group in various time points (P＜0.05).The retina thickness was gradually reduced during the seven-week duration in the diabetic control group (F =91.859,P =0.000),but no significant change was identified in retina thickness in the vitamin D3 group(P＞0.05). Conclusions Vitamin D3 has prevent and therapeutic effects on experimental diabetic oculopathy. Key words: Vitamine D3; Diabetes/experimental; Cornea; Choroid; Retina

Background: Pregnancy-induced hypertension is a serious complication during pregnancy causing maternal and fetal morbidity and mortality. Pregnancy-induced hypertension associated with thrombocytopenia can help in predicting the severity of the disease. The study aimed to study the platelet count, and platelet indices in Pregnancy induced hypertension patients and compare them with normotensive pregnant women. Materials and methods: This was a case control study, conducted on patients visiting antenatal care (ANC) at a Tertiary care rural hospital in western India. Study population included a total of 82 patients, which were divided into 2 groups with 41 patients in each control (healthy pregnant women) and study groups (pregnant women with PIH). Patients with chronic hypertension and known case of thrombocytopenia were excluded from the study. The blood samples collected were processed on fully automated cell counter for estimation of platelet count and indices. Results: The platelet count in the study group ranged from 186434.1±81086.9 whereas in the control group it was 268659±72206.9, showing that the platelet count was significantly lower in women with Pregnancy induced hypertension than in the control group. The MPV, PDW, systolic BP, diastolic BP was found to be significantly elevated in the study group as compared to the control group. Conclusions: The present study concludes that blood parameter such as platelets count and platelet indices can be considered as an early, economical and easily available parameter for assessment of severity of PIH cases.

Introduction: Type 2 diabetes is a chronic disease with warning increasing rate in the world. Although type 2 diabetes is a multifactorial disease, it has been recently found that the incidence of type 2 diabetes is associated with MTNR1B gene and the growth of its expression increases the risk of type 2 diabetes. The objective of this study was to evaluate the effect of 12-week aerobic exercise on MTNR1B gene expression, glucose and fasting insulin in male Wistar rats with type 2 diabetes. Methods: This fundamental study with experimental approach. included 30 male Wistar rats with weight of 220±20 gram. Sampls were kept under standard conditions: temperature of 22 ± 3° centigrade, 45% humidity and consecutive 12-hour periods of light and darkness. The rats were divided into three groups. Infusion of diabetes was conducted by Nicotinamide and streptozotocin solution.Training program was held on 5 days a week for 12 weeks with a gradual increasing of speed (18 to 26 meters per minute) and time (10 to 55 minutes) by running on a treadmill. Glucose level, insulin level and gene expression were measured by enzyme glucose oxidase colorimetric method, enzyme-linked immunosorbent assay, and Real-time polymerase chain reaction. All statistical analyses were performed by software SPSS, version16. Results: Training programme led to a significant reduction in fasting glucose levels in aerobic diabetic group compared to the control diabetic group (P<0.05). Serum insulin levels in the diabetic aerobic group was more than diabetic control group, but this difference was not statistically significant (P≥0.05). On the other hand, the implementation of aerobic exercise MTNR1B in pancreas gene expression decreased 39 percent in the diabetic control group in comparison with diabetic control group (P<0.05). Conclusion: The results showed that 12-week aerobic exercise led to a significant reduction in blood glucose and in gene expression MTNR1B in pancreatic tissue compared to the diabetic control group.

Background: Diabetes mellitus is one of the common medical diseases with pregnancy. Increasing maternal age, overweight, life style change and family history of diabetes are all risk factor for diabetes mellitus. Methods: The objectives of this study are to evaluate the foeto-maternal complications during pregnancy & delivery due to DM/GDM. This prospective study was done in gynae and obstetric dept. of RpMCH during the period of July 2012 to June 2014. The study includes both pre-gestational diabetes & newly diagnosed diabetes as GDM cases admitted through the emergency or OPD with or without labour pain. Results: During the study period total 20302 obstetrics patients were admitted out of them 96(0.47%) were diabetes mellitus with pregnancy. Both age & parity matched study were done between diabetic & control group. Among them 22 (44%) had pre-gestational diabetes mellitus and 28(56%) had gestational diabetes mellitus among the study group 24(48%) were above the age of 30 years, majority were multipara 38(76%) and 24(48%) patients were with the gestational age of 38-40 weeks. Majority patients of diabetic group 28(56%) belongs to average socioeconomic status where as in control group 14(28%) and 20(40%) patients in diabetic group were secondarily educated in comparison to 04(08%) in control group those are statistically highly significant (p=0.000). Regarding BMI 40% were overweight in diabetic group compare to 20% in control group that is statistically significant as a whole (p=0.006). There was no significant difference in the rates of foetal birth asphyxia but macrosomic baby in diabetic group is 16%. Caessarean section were 44(88%) in diabetic group compare to 15 (30%) in control group that is statistically highly significant (p=0.000). Successful vaginal delivery had done in 12% patients in diabetic group under supervision. Conclusion: Hospital delivery mandatory preferably in tertiary care centre to reduce the foeto-maternal complications.

Background. Diabetes mellitus is a condition that causes biochemical and physiological changes in the body. In this study, the effects of carvacrol on blood biochemical factors related to diabetes in diabetic adult rats induced by streptozocin were investigated. Methods. In this study, 32 male Wistar rats were divided into four groups as follows: a) control group; b) diabetic control group that received normal saline as a solvent; c) diabetic rats treated with 75 mg/kg carvacrol; d) healthy rats that received 75 mg/kg carvacrol. The experimental groups were induced with diabetes by injecting a single dose of 50 mg/kg streptozotocin (STZ) (Sigma) dissolved in 5 ml citrate buffer (pH=4.5) intraperitoneally. Then, fasting blood sugar, serum insulin level, serum level of fat profile, and testosterone hormones LH and FSH were measured. Results. Fasting blood sugar and serum levels of TG and cholesterol increased significantly in the diabetic control group compared to the control group (P&lt;0.05). Also, fasting blood sugar and serum TG, LH, FSH, and cholesterol levels in the groups treated with carvacrol showed a significant decrease compared with the diabetic control group (P&lt;0.05). In all diabetic groups, compared to the control group, the amount of HDL, testosterone, and serum insulin decreased significantly (P&lt;0.05). Also, in the groups treated with carvacrol compared to the diabetic control group, the levels of HDL, insulin, and serum testosterone increased. The weight of rats in the diabetic control group decreased significantly; however, in the carvacrol treatment groups, the weight of the rats increased compared to the diabetic control group (P&lt;0.05). Conclusion. Carvacrol can be effective on fasting blood sugar, insulin serum level, serum level of fat, and hormone profile and prevent biochemical damage caused by diabetes in diabetic rats induced by streptozocin. Practical Implications. Investigating the level of lipid profile and hormones in diabetic rats treated with carvacrol.

This study aims to investigate the relationship between thyroid peroxidase antibody (TPO) and thyroid function tests (TSH, T3 and T4) in patients with type 2 diabetes mellitus (T2DM). Ninety women and men, with ages ranging between 35-65 years and weighing 60-80 kgs, were selected for this study. They were classified into three groups: G1 included 15 healthy control group, G2 had15 patients with T2DM and G3 had 60 patients with T2DM and hypothyroidism. Blood samples were collected from each individual via vein puncture to assess thyroid hormone and TPO-Ab. The results showed highly significant (p &lt; 0.01) increase in TSH level in the diabetic group with hypothyroidism when compared to the other groups. There was no significant difference between diabetic and control groups. T4 and T3 levels showed highly significant (p&lt;0.01) decrease in the diabetic group with hypothyroidism when compared to the diabetic and healthy control groups. On the other hand, there was no significant difference between the diabetic and healthy control groups. The results showed highly significant (p &lt; 0.01) increase in the TPO-AB level in diabetic group with hypothyroidism when compared with the diabetic and healthy control groups.

Objective: To investigate the intestinal flora in elderly patients with type 2 diabetes mellitus with early nephropathy. Methods: 43 elderly patients with type 2 diabetes mellitus with early nephropathy (diabetic nephropathy group) and 51 elderly patients with type 2 diabetes mellitus (type 2 diabetes mellitus group) admitted to our hospital from January 2021 to October 2022 were retrospectively analyzed, with 39 healthy people who underwent a physical examination in our hospital during the same period as the control group. The fecal specimens of the three groups were collected, and the 16S rDNAs of bacteria in the fecal samples were extracted, amplified, and sequenced for intestinal flora operational taxonomic unit (OTU) classification and Alpha diversity analysis. Results: (1) Intestinal flora OTUs: there were 545 intestinal flora OTUs unique to the control group, 424 intestinal flora OTUs unique to diabetic nephropathy, and 321 intestinal flora OTUs unique to the type 2 diabetes group. There were 403 intestinal flora OTUs common to the control group and diabetic nephropathy group, 256 intestinal flora OTUs common to the control group and type 2 diabetes group, and 298 intestinal flora OTUs common to the type 2 diabetes group and diabetic nephropathy group. 235 intestinal flora OTUs were common to all 3 groups of subjects. (2) Alpha diversity: The statistical analysis indicated that there was a statistically significant difference (P &lt; 0.05) in the Alpha diversity of intestinal flora, as assessed by the Ace index and Simpson’s index, among the three subject groups. However, no statistical significance (P &gt; 0.05) was observed when comparing the Chao 1 index and Shannon index. Further observation of the Ace index and Simpson index in the three groups revealed that both the diabetic nephropathy group and the type 2 diabetes mellitus group had lower values than the control group. Conclusion: The diversity of intestinal flora decreases in elderly patients with type 2 diabetes mellitus with early nephropathy.

Aims: Diabetes mellitus, a condition characterized by glucose receptor abnormalities affecting glucose uptake, affects approximately 600 million individuals globally as of 2021. This study aimed to assess the antilipidemic and renal effects of aqueous extracts from the root bark and flowers of Terminalia catappa on streptozotocin-induced diabetic rats. Methodology: Twenty-five Albino rats weighing 160 – 300g were divided into five groups: A - normal control, B - diabetic control, C - diabetic treated with root bark extract, D - diabetic treated with flower extract, E - diabetic rats treated with glibenclimide. Diabetes was induced using streptozotocin (55mg/kg). The extracts (200mg/kg) were orally administered for 14 days, after which lipid profiles, renal and liver function tests were conducted. Results: There was a significant (p&lt;0.05) increase in total cholesterol, serum liver enzymes, and kidney markers in the diabetic control group compared to the normal control group. Treatment with the Terminalia catappa extracts for 14 days resulted in more than 20% decrease in urea (from 18.58 Mmol/L to between 11.58 and 13.92 Mmol/L), creatinine (from 343.56 Mmol/L to between 223.94 and 266.30 Mmol/L) and uric acid (from 570.54 µmol/L to between 413.55 and 440.62 µmol/L) concentrations by more than 20%, with the root bark extract showing the most significant effect. Additionally, the Terminalia catappa extract-treated groups exhibited a substantial (around 40%) reduction in serum liver enzymes compared to the diabetic control group. The hepatoprotective capacity of the root bark extract was similar to the glibenclimide-treated group. Furthermore, the extracts led to a 3% reduction in total cholesterol, triglycerides, and low-density lipoproteins, along with a significant increase in high-density lipoproteins. Conclusion: The aqueous root bark and flower extracts of Terminalia catappa demonstrate potentials for managing diabetes mellitus at the specified dosage.

The study was carried out to investigate the anti-diabetic effects of two medicinal plants Cataranthus roseus (C.R) and Nauclea latifolium (N.L) on some biochemical indices of streptozotocin induced diabetic albino wistar rats.&#x0D; Methods: Ethanolic leaf extracts of C.R. and N.L. were given at daily doses of 500 mg/kg body weight in two divided doses each for 14 days. Thirty albino wistar rats were divided into five (5) groups, consisting of 6 rats each viz: Group 1(normal control), Group 2 (diabetic control), Group 3 (insulin treated), Group 4 (received N.L) and Group 5 (received C.R.).&#x0D; Results: The results of the phytochemical screening contain flavonoids, polyphenols, and alkaloids were found to be present in appreciable amount in N.L while saponins and tannins were found in traceable concentration. Fasting blood glucose levels showed significant decrease (P&lt;0.05) in all the test groups compared to diabetic control and closely related to the insulin treated groups. A significant increase (P&lt;0.05) was observed in (TG) and (TC) concentration of all treated groups compared to the diabetic control group. The concentration of HDL was significantly increased while there was also a significant decreased (P&lt;0.05) in VLDL and LDL in the diabetic control group and insulin group when compared to the normal control group, except for C.R treated group that shows a significant decrease compared with the diabetic control group. Enzymes activities were increased in insulin and diabetic groups. A significant reduction (P&lt;0.05) was observed with the treated group of C.R. and N.L compared to the normal control group. Also, observed was a decrease in albumin level in groups treated with the extracts. Marked reduction in total protein level was observed in groups treated with extracts and insulin, compared to the normal control group. Serum concentrations of Na+, K+, Cl- in diabetic control groups showed a significant increase (P&lt;0.05) compared to the normal control group. K+ concentration was observed to be significantly decreased (P&lt;0.05) in all groups treated with extract and insulin compared to the normal control group.&#x0D; Conclusions: The results demonstrated that C. roseus and N. latifolium have anti-diabetic and antihepatotoxic properties and could be potential herbal remedy in treating and managing diabetic conditions.

Objective To investigate the effects of docosahexaenoic acid (DHA) on large conductance Ca2+- activated K+ channel (BK channel) open probabilities (NP0) in coronary smooth muscle cells of diabetic rats. Methods Streptozotocin-induced diabetic rat animal model was established by injection intraperitoneally. Male SD rats (n=20) with weight of (200±20) g and age of 6-8 weeks were divided into diabetic group (n=10) and normal control group (n=10) by random number table method. Diabetic group was injected intraperitoneally with streptozotocin. Blood glucose was tested after 2 weeks. If it was less than 300 mg/dl, same dose streptozotocin was injected intraperitoneally again. Diabetes was diagnosed until blood glucose was more than 300 mg/dl for 8 weeks. The control group was injected by normal saline. Coronary smooth muscle cells in control and diabetic rats were isolated immediately by enzyme digestion method. The BK currents in coronary smooth muscle cells of control and diabetic group were recorded by single channel patch-clamp technique. Effects of DHA on NP0 of BK channels in coronary smooth muscle cells of control and diabetic rats were compared. Studentt test or Wilcoxon rank test were used to compare the NP0 between control and diabetic groups. One-way ANOVA or M test were applied to compare the NP0 in different DHA concentrations groups. Results When test potentials were at 0, 20, 40, 60, 80, 100, 120 mV, NP0 of BK channels increased with test potentials in both control and diabetic group (F=15.28, 9.72, allP 0.05). At 3, 5 and 10 μmol/L, DHA activated BK channels in a dose- dependent manner in both control group and diabetic group (F=10.21 ,7.32, allP<0.05). The NP0 were lower in diabetic group than in control group at the same DHA concentrations (t=2.71 - 8.54, allP<0.05). Conclusion BK tunnel capacity of the coronary smooth muscle cells in diabetic rats was impared. The protective effects of DHA on diabetic heart may be performed by activating BK channels, increasing NP0 and dilating coronary arteries. Key words: Docosahexaenoic acid; Diabetes mellitus; Coronary artery; Large conductance Ca2+-activated K+ channel; Patch-clamp

Introduction. The purpose of this study was to investigate effects of surface treatment of mini-implants in diabetes-induced rabbits by comparing osseointegration around mini-implants. Methods. Twelve New Zealand white rabbits were divided into two groups (alloxan-induced diabetic group and control group). A total of 48 mini-implants were placed after four weeks of diabetic induction. 24 mini-implants were surface-treated with SLA (sandblasted with large grit, and acid etched) and the remaining 24 mini-implants had smooth surfaces. Four weeks after placement, 32 mini-implants were removed from 4 control and 4 diabetic rabbits. Insertion and removal torques were measured. The remaining 16 mini-implants from the two groups were histomorphometrically analyzed. Results. Maximum insertion torque showed no difference between diabetic and control groups, but total insertion energy was higher in control group. In surface-treated mini-implants, maximum removal torque was higher in both diabetic and control groups. Bone-implant contact (BIC) was increased in the control group when compared to the diabetic group. Surface-treated group had higher BIC than smooth surface group in both control and diabetic groups. However, there was no significantly statistical difference. Conclusions. Type 1 diabetes mellitus and surface treatment method of mini-implant affected primary stability of mini-implants. In addition, the use of orthodontic mini-implants in a diabetic patient is likely to show results similar to the healthy patient.

Background: The genetic susceptibility to IDDM maps to the MHC class II region, thus one approach to investigating the immunopathogenesis of diabetes is to study first degree relatives of affected individuals. Objectives: To detect the inherited relationship between (HLA-DRB1) allels and type one Diabetic mellitus. Methodology: A case-control study was performed on 90 persons, they divided into three group which are diabetic, siblings and control (30 persons in each group), who attended to Al Zahraa Teaching Hospital /Diabetic Center in Al Kut/Iraq between the period of April; 2012 till April; 2013.data was analyzed by using SPSS 16th version. Distribution of HLA-DRB1 loci among the study groups (type 1 Diabetic patients, Siblings and Control group) were performed using MR.SPOT ROBOTING system. Results: results showed that the frequencies of HLA-DRB1*3,*4 {25 (69.4%)} among Diabetic group were Significant (P value = 0.002), in compare to the corresponding frequencies among control group {11 (30.6%)}, in compare to the frequencies of other HLA-DRB1 loci in both Diabetic group and Control group { 15 (34.1%), 29 (65.9%)} respectively. Meanwhile results have showed that there were no Significant (P value = 0.116), in the frequencies of HLA-DRB1*3,*4 {25 (58.1%)} among Diabetic group in compare to the frequencies of HLA-DRB1*3,*4 {18 (41.9%)} among Siblings group, in compare to the frequencies of other HLA-DRB1 loci among Diabetic group and Sibling group {15 (40.5%), 22 (59.5%)} respectively, also results have showed that there were no Significant (P value = 0.104), in the frequencies of HLA-DRB1*3,*4 {18 (62.1%)} among Sibling group in compare to the frequencies of HLA-DRB1*3,*4 {11 (37.9%)} among Control group, in compare to the frequencies of other HLA-DRB1 loci among Sibling group and Control group {22 (43.1%), 29 (56.9%)} respectively. conclusion: In conclusion there were a genetic predisposition of diabetic Siblings for development of Diabetes since, both Diabetic group and Sibling group showing the highest frequencies of HLA-DRB1 *3,*4, in compare to Control group. Recommendation: larger sample group are required to include other allels

Objective To study the effect of diabetes on spatial memory ability and spatial associative memory ability in rats. Methods 70 SD rats( 180±20 g) were randomly divided into 3 groups: control group,type1 diabetes group and type2 diabetes group. Type1 and type2 diabetic rat models were set up by streptozocin (STZ) intraperitoneal injection and high fat forage raise. The blood glucose was determined. After rat diabetic model established 1 month and 3 months, respectively, the Morris water maze experiments were implemented,including 4 days' place swimming and 1 day's space exploration. Results After 1 month, diabetic rats' spatial memory ability and spatial associative memory ability were not affected. After 3 months, in place swimming test,the escape latency of two diabetic rat groups was obviously longer than that of the control group (P＜0.05). From the second day of the experiment, escape latency of the control descended sharply, while that of the diabetic group descended slowly. There was no difference between type 1 and type2 diabetic groups in escape latency (P＞0.05). After 3 months, in the space exploration test, when rats were put into the maze from I quadrant which already trained, swimming time in the platform quadrant was shorter, the other parameter scores were lower of the two diabetic model groups, contrast to the control group (P＜0.05). The parameter scores of type2 diabetic group were lower slightly than type1 diabetic group. When rats were put into the maze from IV quadrant for which never trained, the parameter scores of two diabetic groups were lower than that of the control group (P＜0.05) and the total score of type 1 group was lower than that of type2 group. Conclusion The spatial memory ability and spatial associative memory ability of type 1 and type2 diabetic rats descended. In the experiment, the spatial memory ability of type2 diabetic rats was more significantly affected than that of type1 diabetic rats. By contrast,type 1 diabetic rats' spatial associative memory ability descended greatly than that of type2 diabetic rats. Key words: Type1 and type2 diabetes; Rats; Morris water maze; Spatial memory ability; Spatial associative memory ability

ANALYSIS PLATELET INDICES IN PATIENT TYPE 2 DIABETES MELLITUS WITH VASCULAR COMPLICATION Nelly1, Suci Aprianti2 ,Darmawaty ER31Medical Doctor Specialist Education Program of Clinical Pathology, Faculty of Medicine, Hasanuddin University/Dr. Wahidin Sudirohusodo hospital, Makassar2Departement of Clinical Pathology, Faculty of Medicine, Hasanuddin University3Departement of Clinical Pathology, Faculty of Medicine, Hasanuddin University /Dr. Wahidin Sudirohusodo Hospital, Makassar ABSTRACT Introduction :Type 2 Diabetes Mellitus(T2DM) is an endocrin disease that is marked by hyperglycemic condition due to a decrease increase in insulin secretion and insulin resistance or both. Indonesia is the 7th country with the highest incidence of diabetes mellitus in the world. Progression of the disease is slow and cause vascular endothelial damage. The increase of platelet indices is suspected to be an indications of vascular complicationsObjective :To evaluate the platelet indices in diabetics patients with complication and correlate these indices with GDP and HbA1c. Materials and methods :We analyzed platelet indices and biochemical data of patients seen in outpatients by clinicans of wahidin soedirohusodo hospitals. 120 patients with type 2 diabetes mellitus were included with 64 patients vascular complication and 56 patients without complication.Result :We observed an increase in mean platelet volume (MPV): 9,96 + 1.99vs 8.87 + 1.35(p-value: 0,001) ;in platelet distribution width (PDW): 15.29 + 3.45 vs 12.36 + 2.819(p-value: 0,000; without increase plateletcrit (PCT); 0.28 + 0.14 vs 0.25 + 0.12 (p-value: 0.168). we observed a correlation between FBG with PDW (P=0.044).Conclusion :The study findings point to the significant differences in platelet indices (MPV and PDW) in patients with T2DM with complication and without complication, suggesting the presence of platelet in diabetics type 2 patients more reactive and aggregatable in this group of individuals. These suggest that platelet evaluation may be useful in the early detection of long term complication in diabetics patients.Keyword :T2DM, platelet indices, complication vascular