Abstract
The antibiotic jinggangmycin (JGM) is an agrochemical product widely used in China for controlling rice sheath blight, Rhizoctonia solani. Unexpectedly, it stimulates reproduction in the brown planthopper (BPH), Nilaparvata lugens (Stål). However, the underlying molecular mechanisms of the stimulation are unclear. The present investigation demonstrates that adipose triglyceride lipase (Atgl) is one of the enzymes involved in the JGM-stimulated reproduction in BPH. Silence of Atgl in JGM-treated (JGM + dsAtgl) females eliminated JGM-stimulated fecundity of BPH females. In addition, Atgl knockdown significantly reduced the protein and glycerin contents in the ovaries and fat bodies of JGM + dsAtgl females required for reproduction. We conclude that Atgl is one of the key enzymes responsible for JGM-stimulated reproduction in BPH.
Highlights
brown planthopper (BPH) is a serious rice pest in Asia and China[7,8,9]
The present study investigated differentially expressed genes in JGM-treated adult BPH females based on the gene expression profiles, and identified adipose triglyceride lipase gene (Atgl) as the target for gene silencing by RNAi because it was highly expressed in JGM-treated females and because it plays critical roles in energy balance leading to reproduction
We targeted Atgl for RNAi analysis of the JGM-induced molecular influence on the reproduction and biochemistry of BPH because Atgl was upregulated by 3.3 folds in the JGM-treated females compared to that in the control females and because it plays an important role in the energy metabolism pathway required for reproduction (Fig. 1)
Summary
But not all, pesticides stimulate BPH reproduction[2,10,11] and result in a resurgence of its occurrence[12,13]. The present study investigated differentially expressed genes in JGM-treated adult BPH females based on the gene expression profiles, and identified adipose triglyceride lipase gene (Atgl) as the target for gene silencing by RNAi because it was highly expressed in JGM-treated females and because it plays critical roles in energy balance leading to reproduction. Atgl plays an important role in the maintenance of energy balance by regulating triglyceride decomposition and is an ideal RNAi target for investigating its role in JGM-stimulated BPH reproduction. The objectives of the present study were to examine the molecular evidence that JGM stimulated reproduction in BPH and to provide new insights into the resurgence of this pest
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