Abstract
After the incubation of slices of human skin with adenine-2,8- 3 H in the presence of theophylline, 3 H was found in cAMP, indicating the presence of adenyl cyclase in human skin. This activity was stimulated by epinephrine and NaF. Specimens of psoriatic skin were significantly less effective in incorporating adenine-2,8- 3 H into camp, less responsive to the stimulation of epinephrine and unresponsive to NaF. The unaffected skin of the psoriatic patients had normal activity and normal responses to epinephrine and NaF. The 650 × g pellets from homogenates of unaffected skin and affected skin from apsoriatic patient were prepared and incubated with 2 mM ATP-α- 32 P. The amounts of cAMP- 32 P formed were compared. The preparation from the unaffected skin produced 7.5 pmoles of cAMP per min per mg protein, while the affected skin had approximately half the capability. These results suggest deficiency and defects of adenyl cyclase in psoriatic skin.
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