Abstract
Objective To study the protective effect of adenovirus-mediated heme oxygenase-1 (HO-1) transfection on the spinal cord ischemia-reperfusion injury (IRI) in rats.Methods SD rats were randomly divided into sham operation group (group A),ischemia reperfusion group (group B) and AdV-rHO-1 group (group C),The rats in groups B and C were given spinal cord ischemia for 30 min.Physiological saline,AdV-EGFP,and AdV-rHO-1 were injected in groups A,B and C,respectively.Hind legs nerve function grading,apoptotic cells,immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were used to evaluate the degree of spinal cord injury.Results The hind legs nerve function grading (2.83 ± 0.75) in group C was obviously better than in group B (1.5 ± 0.55) after reperfusion for 2 days (P ＜ 0.01).The histopathologic changes in group C were obviously milder than those in group B.Immunohistochemistry and RT-PCR indicated that transgene was already expressed in group C.The level of HO-1 in group C (35.85 ±6.59) was higher than that in group A (7.24 ± 1.15) or group B (8.21 ± 3.05) (P ＜ 0.01).Conclusion Adenovirus-mediated gene transfection of rHO-1 has significantly protective effect on spinal cord IRI. Key words: Gene transfection ; Heme oxygenase-1 ; Adenovirus ; Spinal cord ischemia; Reperfusion injury
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