Abstract
A complete protocol is presented for in vitro regeneration of Milletia pinnata (L.) Panigra-hi, a biodiesel producing medicinal tree, using cotyledonary nodes derived from axenic seed-lings. Shoot development was induced from cot-yledonary nodes on Murashige and Skoog (1962) medium supplemented with 8.88 and 11.1 μM benzyladenine (BA) with or without ad-ditives (silver nitrate (AgNO3) and/or adenine sulphate (Ads)). The maximum number of shoot bud (24.33 buds/explant) and elongated shoots (8.33 shoots/explants) was developed on a medi-um containing 8.88 μM BA with 11.84 μM AgNO3 and 108.6 μM Ads. Upper 2.0 cm portion of elon-gated shoot was harvested for rooting and re-maining portion was cut into nodal segments and cultured on a fresh medium for further mul-tiplication. Thus, from a single cotyledonary node about 40–45 shoots were obtained within eight weeks of culture. Sufficiently long shoots were excised and rooted on half-strength MS supple-mented with 9.84 μM indole butyric acid (IBA). Plantlets were successfully acclimated and estab-lished in soil.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Vegetos- An International Journal of Plant Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
