Abstract
Abstract We have demonstrated previously that fluid secretion rates of Malpighian tubules from larval Aedes aegypti are stimulated by a diuretic peptide and by 5-hydroxytryptamine (5-HT, serotonin). In the present manuscript we demonstrate that diuretic peptide and 5-HT have additive effects on Malpighian tubule fluid secretion rates at low 5-HT concentrations (1–100 nM), while Malpighian tubules stimulated by higher 5-HT concentrations (1–10 μ M) do not respond to diuretic peptide in the fluid secretion bioassay. Fluid secretion rates are stimulated by forskolin and dibutyryl cAMP, but are unaffected by IBMX. Cyclic AMP concentrations are stimulated by 5-HT in vitro , while diuretic peptide has no effect on cAMP concentrations. These effects strongly support stimulation of adenylate cyclase activity by 5-HT and suggest that 5-HT and diuretic peptide act through separate second messenger systems.
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