Abstract
A commercial ion-exchange column method (Quik-Sep) for isolation of immunoglobulin M (IgM) free from immunoglobulin G (IgG) was evaluated. After column separation, serum IgM recovery averaged 88% (46-100%) with IgG removal averaging 95% (91-100%). Rubella and Toxoplasma gondii IgM antibodies were recovered without a change in titer, whereas IgG antibodies were removed effectively. Rheumatoid factor (RF) interference and IgG blocking antibodies were eliminated following column chromatography.
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