Abstract
Breast cancer takes the first place in the structure of oncologic morbidity of female population of the planet. Potential targets in elaboration of drugs are cysteine proteinases taking part in many processes, associated with cancer progression: Hyperproliferation of tumor cells, apoptosis, tumor-induced angiogenesis, invasion and matastasis.Cathepsin H cysteine proteinase possesses both proteinase and exopeptidase activity. High cathepsin H activity has been determined in the following tumors: glioblastoma, anaplastic astrocytoma, colorectal carcinoma, in head and neck tumors in blood plasma of patients with lung cancer, melanoma.In this connection the aim of the work consisted in analysis of cathepsin H activity in the tissue of invasive lobular form of breast cancer in order to elaborate additional biochemical test for presence of a neoplasm.Samples of mammary gland without neoplasms (circumferential tissue) and samples with invasive lobular form of breast cancer at I-II stages of cancer development were analysed. The patients were informed and gave the written agreement for the use of the anatomical material for biochemical research. In the supernatant the content of protein was determined by biuret method and cathepsin Н activity was determined by the modified Bradshaw method. Cathepsin activity was expressed in mmole of leucine, formed in the process of oxytocin hydrolysis, per a mg of protein per a min at 37 °С.It was found that in case of invasive lobular form of BC, the content of protein in tumour tissue at all stages of its development was 1.6-2.6 higher than the content of protein in the circumferential tissue. It was shown that cathepsin Н activity changed depending on the stage of invasive lobular BC development. The maximal relative and specific activity of cathepsin Н in tumour tissue was determined at I-II stage of tumor development. With tumor progression relative and specific activity of cathepsin Н in tumour tissue
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