Abstract
Neutral red is mainly used to identify and detect the activated state of cells, which accumulates in the lysosomes of uninjured cells. In this paper the influence factors on microalgae of neutral red concentration, staining time, algal cell concentration and pH were studied, which revealed the optimized conditions. The maximum staining ratio occured after 8 min staining with 0.22 g•L-1 of neutral red at pH 7.3. The algal cell concentration had no obvious effect on staining ratio. The UV and UV/O3 process were also investigated for ballast water treatment using heterosigma akashiwo as an indicator. Compared with individual unit processes using UV, the inactivation efficiency of heterosigma akashiwo by the combined UV/O3 process was enhanced. The algae after treatment was unable to re-grow and it revealed that the algal activity assays can reflect the inactivation effect more correctly than cell density, which suggest the neutral red staining method has potential for viability detection of microalgae in ship’s ballast water.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
