Abstract
The structure and biosensor characteristic of complex between phenoloxidase (tyrosinase) and phoshpholipid thin ordered layer were investigated. The advantageous of immobilization method based on Langmuir–Schaefer (LS) film as a matrix has been developed for characterization of sensing protein layers effect. Tyrosinase from Agaricus bisporus molecules were densely adsorbed onto Langmuir monolayer surface and then deposited on solid support which can be used for phenolic compounds determination. The enzyme was immobilized by adsorption process. Phenoloxidase was adsorbed on monolayer built of 1,2-dipalmitoyl- S,N-glicero-3-phosphocholine (DPGPC). The sensor sensitization was achieved by an amphiphilic conducting structure – N-hexadecyl-3,6-bis(thianthrene)carbazole (HBTC) admixed into the film. The interlaced compound was expected to facilitate the electron transfer as well enhancing the sensor sensitivity. Considering the fact, that immobilization strategy showed high efficiency, obtained results suggest that our method for tyrosinase immobilization has a great potential of enabling high throughput fabrication of bioelectronics’ devices.
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