Abstract
Abstract In the course of our work on the formation and properties of the EAC1̄,,5b,6 intermediate, we have found that eluates prepared from these cells contain a complex factor which has an apparent sedimentation rate of 14S to 15S and comprises C5b and C6. This material, designated C5b/6, converts EAC ∼ 3 (EAC or EAC4,3b) to EAC ∼ 6 (i.e., an intermediate susceptible to lysis by C7, C8 and C9). The conversion proceeds in the presence of EDTA. In contrast, native C5 and C6 will react only with EAC to yield EAC ∼ 6, i.e., cell-bound C2a is essential for the reaction with native C5 and C6. The lack of C2a requirement in the reaction with C5b/6 is supported by the observation that monospecific rabbit anti-guinea pig C2 does not inhibit C5b/6 actionon EAC ∼ 3. On the other hand, as would be expected, monospecific rabbit anti-guinea pig C5 effectively blocks conversion of EAC ∼ 3 to EAC ∼ 6 by C5b/6.
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