Abstract
In this study, two purified polysaccharide fractions, Artp1 and Artp2, were obtained using acid-catalyzed water extraction, and then purified by DEAE-52 cellulose and Sephadex G-200 column chromatography from the crude polysaccharides of Artemisia argyi. Their physicochemical properties were investigated by gel permeation chromatography (GPC), high-performance anion exchange chromatography (HPAEC), Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermal analysis, and methylation analysis. The average molecular weight (Mw) of Artp1 and Artp2 were estimated to be 42.17 kDa and 175.22 kDa, respectively. Monosaccharide composition analysis revealed that the Rha, Gal, and GalA occupied main proportion in Artp1 with the molar ratio of 25.1:24.7:40.4, while the Rha, Gal, Xly, and GalA occupied the main proportion in Artp2 with the molar ratio of 16.7:13.5:12.8:38.7. Due to the high yield and the relatively high carbohydrate content, the Artp1 was determined by the methylation analysis and NMR. The results of Artp1 indicated that 1,4-GalpA and 1,2,4-Rhap formed the backbone with some 1,2-Rhap, 1,3-Galp, and 1,6-Galp in the backbone or the side chains. Artp1 and Artp2 exhibited effective antioxidant activities by DPPH radical scavenging assay and hydroxyl radical scavenging assay in a dose-dependent manner. These investigations of the polysaccharides from A. argyi. provide a scientific basis for the uses of Artp1 and Artp2 as ingredients in functional foods and medicines.
Highlights
Artemisia argyi (A. argyi), known as “Aicao”, is a perennial herbaceous plant widely distributed in China for a long history
The pre-treatment result indicated that the yield of A. argyi polysaccharide was not very well obtained by traditional hot water extraction
Its yield was 14.06% of raw material powder, which is higher than that extracted by hot water [3]. This could be attributed to the reason that the structure of the cell wall could be broken down, causing polysaccharides in the cell to be diffused into the solvent
Summary
Artemisia argyi (A. argyi), known as “Aicao”, is a perennial herbaceous plant widely distributed in China for a long history. It is a popular and traditional medicinal and edible plant to treat dysmenorrhea, abdominal pain, inflammation, microbial infections, hepatitis, malaria, cancer, and circulatory disorders [1,2,3]. North A. argyi, distributed in Tangyin County, Henan Province, is called “Jiu Tou Xian Ai” because of the nine veins in its leaf. Due to its excellent quality and profoundly cultural heritage of the origin, North A. argyi is a “national geographical indication protection production of China” [4]. In Tangyin County, the planting area is about 1000 hectare in 2018
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