Acid Hydrolysis Sorghum Straw for Bioethanol Fermentation of <i>Saccharomycescerevisiae</i> TISTR 5596

Abstract

In previous work, Saccharomycescerevisiae TISTR 5596 could produce bioethanol in the strength condition. Thus, this work was examined to understand effect of sulfuric acid on hydrolysis of synthetic cellulose and sorghum straw. The ethanol fermentation of S.cerevisiae TISTR 5596 of the products from the hydrolysis was also determined. The results showed the maximum amount of sugar from hydrolysis reaction of synthetic cellulose in 5%v/v sulfuric acid which are analyzed by high performance liquid chromatography which has yield 28 g/L and 25.46 g/L for the maximum amount of sugar form hydrolysis of sorghum straw in 3%v/v sulfuric acid. Furthermore, the ethanol fermentation at 30°C for 25 hrs with hydrolysis of the synthetic cellulose and sorghum straw was determined by gas chromatography. S.cerevisiae TISTR 5596 can produce 9.021 g/L of ethanol or 32.89% of initial sugar concentration at 28 g/L for fermented at 30°C for 25 hrs. and 1.8 g/L or 22.52% of initial sugar concentration at 25.46 g/L at 30°C for 40 hrs, for extracted samples from synthetic cellulose and sorghum straw, respectively.