Abstract
This chapter describes the acetylcholine (ACh) release from the brain. Considerable information about the release of ACh from the surface of the brain has been obtained using the collecting cup technique. The release of ACh from the region of single cortical neurons has been studied using concentric glass micropipettes, and the original technique has been described briefly. The central barrel of the micropipette is used to record unit electrical activity while fluid is slowly withdrawn up the outer barrel. Several methods have been used to draw small volumes of fluid up the pipette. The resting release was similar using ether, cyclopropane, sodium pentobarbitone, and allobarbitone, but was depressed below the level of detection by chloralose. The depth of anesthesia affected the resting release of ACh, the highest outputs being obtained during light anesthesia or during mild convulsions induced by leptazol. The cortical origin of the released ACh has been established by several methods including undercutting of the cortex, collection of ACh with push-pull cannulae, and analysis of venous effluent from the cortex.
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