Abstract
Sucralose is a non-nutritive artificial sweetener used in a broad range of foods and beverages. In the present study, Bacillus amyloliquefaciens WZS01 was isolated, identified, and used as a catalyst both in regioselective acylation and deacetylation for sucralose preparation. Bacterial cells were immobilized on polyurethane foam and utilized to synthesize sucrose-6-acetate regioselectively. The yield of sucrose-6-acetate was >95% with 60mM sucrose after 22h of reaction. Free cells could hydrolyze 75mM sucralose-6-acetate to produce sucralose with >99% yield after 24h of reaction. B.amyloliquefaciens WZS01 could be considered a potential biocatalyst for sucralose preparation.
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