Accuracy and precision comparison of the hemocytometer and automated cell counting methods

Abstract

The hemocytometer is the most widely used device for determining cell concentrations, requiring consistent criteria and tenacity to obtain measurements correctly and reproducibly. As an alternative, the Countess(tm) Automated Cell Counter employs digital imaging and an image analysis algorithm to identify and enumerate cells in a sample. To compare the performance of these two methods, the accuracy, precision, and effective ranges were determined for total cell count and percent viability. First, using a standardized bead solution, the accuracy and precision of glass and disposable hemocytometers were measured and found to be 30% and 5% different from the anticipated value, respectively. Subsequently, disposable hemocytometers and the automated counter were used to determine total cell numbers and percent viability for multiple cell types over a range of cell concentrations. Using a Student's t‐test, the results showed the Countess(tm) instrument measured cell concentrations and viability as accurately and precisely as the disposable hemocytometer. Additionally, the effective concentration range for the Countess(tm) instrument was two times greater than the hemocytometer, and the viability range matched the hemocytometer. The Countess(tm) Automated Cell Counter overall produces results much more rapidly without the problem of operator tedium and fatigue or compromised accuracy and precision.