Abstract
A bioactivator from honey, stimulating osmophilic yeasts, was compared with Bios. Complementary fractions from treatment with charcoal were found to exert effects similar to Bios I (inosite) and Bios II of Miller and associates, when tested with the Toronto strain of Saccharomyces cerevisiae. Charcoal treatment of honey removes by adsorption Bios II leaving a residue, relatively inert by itself, containing inosite. Inosite, however, does not appear as the active substance in the charcoal filtrate for the strain of osmophilic Zygosaccharomyces tested, the growth of this organism being dependent upon the presence of another substance which, though not essential for the Toronto yeast, appears to be present in crude Bios II.
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