Abstract P2-06-12: The expression of aldehyde dehydrogenase1 in cytoplasm or stroma of breast cancer is associated with clinical prognosis

Abstract

Abstract Background: Previous studies have shown that ALDH1 expression in breast tumor cells was associated with poor prognosis.Whether tumor microenvironment affect prognosis of breast cancer patients is unknown by far. Aim: We performed a research to study whether ALDH1 expression in cytoplasm or stroma of breast tumors is associated with prognosis of breast cancer patients. Methods: As many as 160 cases with invasive carcinoma were retrieved.ALDH1 staining was respectively assessed on cytoplasmic and stroma of tumour cells.Chi-square test was used to evaluate the associations between ALDH1 expression and clinical parameters.Cox proportional hazards analysis was used for relapse-free survival(RFS). Results: There was significant association between ALDH1 expression in stroma and Age,ER. Association between ALDH1 expression status and clinicopathological parameters ALDH1 expression in cytoplasm ALDH1 expression in stroma NPositive (%)Negative (%)PPositive (%)Negative (%)PALL CASES 56 (35)104 (65) 64(40)96 (60) Age (years) 0.867 0.022≤509432 (34.0)62 (66.0) 30 (31.9)64 (68.1) >506624 (36.4)42 (63.6) 34 (51.5)32 (48.5) Tumor size 0.430 0.188≤25417 (31.5)37 (68.5) 18 (33.3)36 (66.7) 2-510038 (38.0)62 (62.0) 46 (46.0)54 (54.0) >561 (16.7)5 (83.3) 1 (16.7)5 (83.3) Number of metastatic lymph nodes 0.452 0.364≤38027 (33.8)53 (66.2) 34 (42.5)46 (57.5) 4-95718 (31.6)39 (68.4) 19 (33.3)38 (66.7) >92311 (47.8)12 (52.2) 11 (47.8)12 (52.2) ER in primary breast tumor 0.156 0.025Positive10934 (31.2)75(68.8) 37 (33.9)72 (66.1) Negative5122 (43.1)29 (56.9) 27 (52.9)24 (47.1) PR in primary breast tumor 0.591 0.598Positive11241 (36.6)71 (63.4) 43 (38.4)69 (61.6) Negative4815 (31.3)33 (68.7) 21 (43.8)27 (56.3) HER-2 in primary breast tumor 0.460 0.585Positive4317 (39.5)26 (60.5) 19 (44.2)24 (55.8) Negative11739 (33.3)78(66.7) 45 (38.5)72 (61.5) Significance level of p<0.05 was considered to be statistically significant. In multivariate analyses of RFS,ALDH1 expression in cytoplasm is associated with a worse prognosis.However,ALDH1 expression in stroma is not related with prognosis. Univariate and multivariate analyses of RFS Univariate Analysis Multivariate Analysis CharactersOR95% CIPOR95% CIPAge ≤50/>500.8120.436-1.5150.513 Tumor size ≤2/>22.4731.144-5.3430.0212.0350.938-4.4170.072Number of metastatic lymph nodes ≤3/>36.3462.815-14.306<0.0015.6552.477-12.910<0.001ER in primary breast tumors -/+0.6600.354-1.2310.191 PgR in primary breast tumors -/+0.5330.288-0.9880.0460.8350.358-1.9480.676HER-2 in primary breast tumors -/+0.9690.487-1.9280.929 ALDH1 in primary breast tumors -/+2.5781.403-4.7370.0022.7741.502-5.1240.001ALDH1 in primary breast stroma -/+0.7120.356-1.4730.378 Significance level of p<0.05 was considered to be statistically significant. Conclusions: We conclude that ALDH1 expression in stroma might suggest the presence of Cancer stem cells in tumor microenvironment.The ALDH1 expression in cytoplasmic of breast tumors rather than in stroma was an independent factor for prognosis. Key words:Breast cancer stem cell;ALDH1;Tumor microenvironment. Citation Format: Xin Guan, Yi Dong, Aiping Shi, Zhimin Fan. The expression of aldehyde dehydrogenase1 in cytoplasm or stroma of breast cancer is associated with clinical prognosis [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P2-06-12.