Abstract C60: Injectable, biodegradable micro- and nano-particles loaded with prodigiosin-based drug for localized anticancer drug delivery

Abstract

Abstract This paper presents the synthesis and physicochemical characterization of injectable, multi-functional, biodegradable poly (D,L-lactide-co-glycolide) (PLGA)-loaded micro- and nano-particles. These particles were loaded with an anticancer drug from prodigiosin (PG), which was obtained from bacteria, Serratia marcescens subsp. Marcescens. The release of paclitaxel (PT) was also tested as a control. The PG and PT were encapsulated using a single-emulsion solvent evaporation technique with PLGA as a polymer matrix and poly-(vinyl alcohol) (PVA) as an emulsifier. The dependence of particle size and morphology on processing conditions was also evaluated. In vitro release studies were used to elucidate drug loading efficiency, encapsulation efficiency and microparticle morphology using a combination of UV-visible (UV-Vis) spectrophotometry, optical Microscopy, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and atomic force microscopy (AFM). The implications of the results are then explored using MDA-MB-231 cells (breast cancer cells) for the development of injectable, multi-functional, polymeric micro- and nano-particles for the controlled release of cancer drugs and the localized treatment of cancer. Citation Format: John D. Obayemi, Karen A. Malatesta, Olushola S. Odusanya, Danyuo Yiporo, Wei Yu, Kathryn E. Uhrich, Winston O. Soboyejo. Injectable, biodegradable micro- and nano-particles loaded with prodigiosin-based drug for localized anticancer drug delivery. [abstract]. In: Proceedings of the Eighth AACR Conference on The Science of Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; Nov 13-16, 2015; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2016;25(3 Suppl):Abstract nr C60.