Abstract B45: Characterization of human fallopian tube epithelial stem-like cells.

Abstract

Abstract Purpose: Recent studies have suggested a large part of ovarian high grade serous carcinomas arise from the fallopian tube epithelium (FTE). The purpose of this study was to derive the stem-like cells of fallopian tube epithelium (FTESC). Results: Immunohistochemistry of human fallopian tube revealed expression of Pax8 in secretory cells, Tubb4 in ciliated cells and Epcam in all the epithelial cells, whereas Lgr5 was expressed in a small subset of cells in the epithelial layer. The presumed FTESC were cultured from the fallopian tube fimbria epithelium exhibiting a cuboidal cell morphology and could be maintained at a constant proliferation rate up to P9. When cultured on matrigel, these cells formed spheroids with Pax8, Tubb4 and Lgr5 expression. Flowcytometry revealed decreasing expression of EpCAM, CD73, CD133 and CD105, and increasing of Pax 8 secretory cell marker (87% in P6, 78% in P3) with the advance of passage. Adding of estrogen to the culture resulted in an enlargement of cell body (4 nM) and differentiation toward ciliated cells (400 nM). When these FTESC were cultured on the mouse embryonic fibroblast feeder, or mixe-cultured with fallopian tube stromal cells and HUVECs, they formed tubal-like structures mimicking the fallopian tube. Conclusion: We successfully derived a presumed FTESC with self-renewal and differentiation capability. These cells may provide new insight to investigate how FTE regenerate, respond to hormone and ROS injury and subjected to cancer initiation. Citation Format: Dah-Ching Ding, Tang-Yuan Chu. Characterization of human fallopian tube epithelial stem-like cells. [abstract]. In: Proceedings of the AACR Special Conference on Advances in Ovarian Cancer Research: Exploiting Vulnerabilities; Oct 17-20, 2015; Orlando, FL. Philadelphia (PA): AACR; Clin Cancer Res 2016;22(2 Suppl):Abstract nr B45.