Abstract A15: Oncomirs expression profiling in uterine leiomyosarcoma cells

Abstract

Abstract miRNAs act as regulators of gene expression at the post-transcriptional level, playing a key role in several biologic processes; their abnormal expression may to lead a cellular transformation and tumorigenesis. miRNAs are considered as oncomirs when regulated oncogenes or tumor suppressor genes. Here, we aimed to evaluate the expression profile of 84 oncomirs sequences in immortalized cells of myometrium (MM), uterine leiomyoma (ULM), and uterine leiomyosarcoma (ULMS), seeking specific signatures for these cells. MM (PCS-460-011), LM (T HESCs CRL-4003) and ULMS (SK-UT-1 HTB-114) cells line were cultured in specific medium and conditions (ATCC recommendations). Quantitative Real Time - PCR was performed using the MIHS-109ZA-Qiagen 96 wells plate; all data were normalized and analyzed by ΔΔCt method in the pcrdataanalysis.sabiosciences.com/mirna software. Data analysis showed nine miRNAs with significant differential expression in ULM and fifteen in ULMS, using MM cells as reference. Six miRNAs were overexpressed in ULM;among them two had a high fold regulation (miR-205-5p and miR-328-3p). In ULMS six miRNAs exhibited a significant overexpression, with greater fold regulation in miR-148a-3p, miR-202-3p, and miR-203a-3p. We found nine downregulated miRNAs in ULMS and four in ULM. A reverse expression was observed in miR-21-5p and miR-27b-3p in ULM and ULMS; in addition, these miRNAs showed a downregulation in ULMS with high fold regulation. miR-495-3p, miR-27b-3p, and miR-152-3p had a significant downregulated expression in ULMS. Only two miRNAs (miR-31-5p and miR-210-3p) showed downregulated expression in ULM than ULMS. In conclusion, our preliminary approach identified 17 oncomirs with a significantly deregulated expression profile in ULM and ULMS that might lead to alterations in their mRNA-target. Their role in ULM and ULMS may be further investigated. Citation Format: Bruna Cristine De Almeida, Natalia Garcia, Edmund Chada Baracat, Kátia Candido Carvalho. Oncomirs expression profiling in uterine leiomyosarcoma cells [abstract]. In: Proceedings of the AACR International Conference held in cooperation with the Latin American Cooperative Oncology Group (LACOG) on Translational Cancer Medicine; May 4-6, 2017; São Paulo, Brazil. Philadelphia (PA): AACR; Clin Cancer Res 2018;24(1_Suppl):Abstract nr A15.