Abstract 9568: Adipose-Derived Mesenchymal Stem Cells Embedded in Platelet-Rich Fibrin Scaffolds Promote Angiogenesis, Preserve Heart Function, and Reduce Left Ventricular Remodeling in Rat Acute Myocardial Infarction

Abstract

Background: This study tested the hypothesis that autologous adipose-derived mesenchymal stem cells (ADMSCs) embedded in platelet-rich fibrin (PRF) promotes myocardial regeneration and repair after acute myocardial infarction (AMI). Methods and Results: Adult male Sprague-Dawley rats equally divided (n=10/group) into group 1 (sham-operated), group 2 (AMI by ligating left coronary artery), group 3 (AMI+PRF), and group 4 (AMI+PRF-embedded autologous ADMSCs) were sacrificed on day 42 after AMI. Left ventricular (LV) dimension, infarct/fibrotic areas, and distance between I bands were lowest in group 1, highest in group 2, higher in group 3 than in group 4, whereas LV performance and wall thickness exhibited a reversed pattern (p<0.001). Protein expressions of inflammatory (MMP-9, IL-1β), oxidative, apoptotic (Bax, cleaved-PARP), fibrotic (Smad 3, TFG-β), hypertrophic (β-MHC), and heart failure (BNP) biomarkers displayed an identical pattern in infarct/fibrotic areas, whereas protein expressions of anti-inflammatory (IL-10), anti-apoptotic (Bcl-2), anti-fibrotic (Smad1/5, BMP-2) biomarkers, and α-MHC showed an opposite pattern (p<0.01). Angiogenic activities (Small-vessel numbers; C-kit+, Sca-1+, CD31+, SDF-1α+, CXCR4+ cells; protein expressions of SDF-1α, CXCR4, VEGF) were highest in group 4 and lowest in group 1, higher in group 3 than in group 2 (p<0.001). Conclusion: ADMSCs embedded in PRF offered significant benefit in preserving LV function after AMI.