Abstract 6329: Edible Chinese mushroom-derived antioxidant, vialinin A, prevents breast cancer cell growth

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Abstract Breast cancer ranks as the second most prevalent cancer worldwide, impacting millions annually, and stands as the leading cancer affecting women. Neoplasia occurs when free radicals, antioxidants, and reactive oxygen species increase in the cell, which can alter protein function and cause mutations and genomic instability. Current breast cancer treatments typically include chemotherapy and radiation. However, different breast cancer cell types have different antioxidative capacities, which allow them to resist these treatments, thereby reducing the efficacy of these expensive treatments. Several vitamins, antioxidants, and anti-inflammatory compounds have been tested for their effectiveness in preventing breast cancer. However, the role of vialinin A, an antioxidant and ubiquitin-specific peptidase inhibitor derived from edible Chinese mushrooms, is unknown. Therefore, in this study, we examined the chemopreventive effect of vialinin A in preventing breast cancer cell growth and migration. Human breast cancer cells (MCF-7) were treated with vialinin A in a time- and dose-dependent manner. Cell viability assay indicates that vialinin A prevents the growth of breast cancer cells in a dose-dependent manner. Further, vialinin also prevented the EGF-induced invasion of MCF-7 cells in a scratch assay. Vialinin A also prevented the EGF-induced trans-well migration of breast cancer cells. Vialinin A also regulated the growth factor-induced expression of various pro- and anti-apoptotic factors in breast cancer cells. Vialinin A also increased the activation of caspase-3 and cleavage of PARP in growth factor-treated breast cancer cells. Next, we plan to examine the chemopreventive efficacy of vialinin A in a nude mouse xenograft model. In conclusion, our results indicate that vialinin A could prevent breast cancer cell growth and invasion, suggesting its potential development as a chemopreventive drug. Citation Format: Malak Rafik, Afnan Khan, Kota V. Ramana. Edible Chinese mushroom-derived antioxidant, vialinin A, prevents breast cancer cell growth [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 6329.