Abstract 5439: A novel drug sensitivity assay method for 3D cultured cell using an imaging device

Abstract

Abstract Three-dimensional (3D) cultured cells are useful for the physiological study of tumor cells owing to their similar characteristics. Currently, the popular methods for 3D cell culture-for example, collagen gel or the matrix method-involve complicated procedures that often result in experimental errors. We attemted to develop a novel technique for the use of 3D cell culture in a novel drug sensitivity assay using an imaging technique. We used a NanoCulture® plate (NCP) for 3D cell culture of the human breast cancer cell lines BT474 and T47D for forming multicellular spheroids and used these in a drug sensitivity assay for trastuzumab and paclitaxel by employing the imaging device BioStation CT. The NCP has a fine pattern carved on the surface of the wells’ bottoms, which provides scaffolding for cells with expanding pseudopodia. Thus, the cells can move and aggregate to form uniform spheroids on the NCP. These spheroids further migrate and fuse each other during culture. The effects of anticancer drugs-i.e., a decrease in the migration velocity of spheroids and suppression of spheroid fusion in a dose-dependent manner-were estimated using NCP-cultures spheroids analyzed using sequential BioStation CT images. These results were compared to the conventional assay method of ATP quantification with a good agreement. We confirmed the antitumor effect of the drugs on cells seeded in a single well of the 96-well plate by this technique. We expect this method to be useful in research on new antitumor agents and in drug sensitivity tests for individually tailored cancer treatments. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5439. doi:10.1158/1538-7445.AM2011-5439