Abstract 4988: Multiplex immunofluorescence detection of resident memory T cells in solid tumors

Abstract

Abstract Tissue-resident memory T cells (TRM) represent a lineage of T cells localized in peripheral tissues that do not reenter the circulation. Their establishment in the tissue makes them the initial T cell response in local infections and inflammatory conditions. TRM are characterized by the expression of CD103, CD69, and CD49a. TRM play a role in the efficacy of cancer vaccines and have been correlated with improved prognosis and/or survival in a number of cancers. Comparisons of TRM and cytotoxic CD8 T cells in the tumor microenvironment are limited. Due to this, we have developed a Histoprofile TRM multiplex immunohistochemistry panel, validated by in-depth image analysis, for TRM and CD8 T cell profiling. Here we present our in depth multiplex immunofluorescence validation assay including tests of repeatability and reproducibility. Human tissue microarrays of tumors in a range of tissues were investigated with the TRM multiplex panel. After multi-spectral acquisition, the TRM and CD8 T cells could be differentiated in all of the tissue types examined. After delineation by a pathologist, we demonstrate the capability to quantify TRM and CD8 cells within the tumor and stroma compartments. The presented approach has the power to expand the clinical implications of TRM in cancer vaccine and immunotherapy. Citation Format: Elena Baranova, Amanda Finan-Marchi, Manon Motte, Maroua Tliba, Sabine Iglesias, Carole Belda, Alexandra Mace, Sylvie Hunt, Domenico Lazzaro, Jean-Philippe Coton, Renaud Burrer. Multiplex immunofluorescence detection of resident memory T cells in solid tumors [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 4988.