Abstract 4405: Orthogonal detection of thymidine analogue based cell proliferation and apoptosis using click chemistry combined with antibody based progesterone receptor detection in estradiol treated rat model

Abstract

Abstract Detection reagents which can simultaneously measure cell proliferation and apoptosis, or measure chronological changes in cell proliferation over the course of a treatment provide insight into efficacy and mechanisms of treatment on cell cycle and cell heath. Detection reagents which can temporally separate cell proliferation while combined with apoptosis detection provide a powerful new investigative tool. When 5-ethynyl-2′-deoxyuridine (EdU) incorporation is used in conjunction with bromodeoxyuridine (BrdU), cell proliferation measurements are made with orthogonal detection platforms permitting unambiguous identification of proliferation events. In rat mammary epithelial tissue changes in cell proliferation as a result of treatment combined with apoptosis detection demonstrates the value of click chemistry when combined with antibody based detection methods. We use the rat model to show changes in proliferation and apoptosis rates in mammary epithelial tissue, after alteration in hormone treatment. Use of click chemistry labeling in ovariectomized rats shows a decrease in proliferation rate from their basal levels, while estradiol treatment results in an increase in proliferation rate over basal level. Cessation of a 7 day estradiol treatment causes a drop in proliferation rate and an increase in apoptosis which can be efficiently detected using click chemistry. Progesterone receptor levels are measured simultaneously with proliferation rate when click chemistry based detection is combined with antibody based detection methods. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4405.