Abstract 3447: Identification of Gastric Cancer-Specific Helicobacter Pylori Genes Using Suppression Subtractive Hybridization

Abstract

Abstract Helicobacter pylori (H. pylori), a microaerophilic Gram negative bacterium, is known to cause chronic gastritis, peptic ulcer, and gastric cancer. Genes that are present in certain isolates may determine strain-specific traits such as drug resistance. In order to understand the pathogenic mechanisms of gastric diseases, identify the molecule markers of the diseases associated with H.pylori strains, and provide clue for the target treatment of H. pylori related diseases, a subtracted DNA library was constructed from a gastric cancer-associated H. pylori strain and a superficial gastritis-associated H. pylori strain by suppression subtractive hybridization. Differentially expressed genes were identified by dot blot hybridization and DNA sequencing. Twelve high copy genes and 9 low-copy genes were found in gastric cancer-associated strain compare to superficial gastritis strain. These differentially expressed genes were confirmed by PCR analysis of bacteria isolates. Interestingly, PPIase was detected positively in 13 out of 26 H pylori strains derived from gastric cancer patients. In contrast, less than 24% of H pylori strains from superficial gastritis showed positive. Our results suggest that PPIase may represent a novel marker and potential therapeutic target for gastric cancer. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3447.