Abstract
Abstract Tumor microenvironment has important influences on tumor progression. One of the effectors is tumor-associated macrophages (TAMs) that are characterized as M1/M2 by their different immune responses, induced by Th1 and Th2. In our study, we polarized THP-1 into classically (M1) and alternatively (M2) activated macrophages and evaluated some different markers to distinguish M1 and M2 subtype (M2a, M2c). M1 macrophage expressed high IL-1, IL-6, IL-12, IL23, TNF-α, and a surface maker CCR7; M2a and M2c macrophages expressed high IL-10 and a surface maker CD206, but expressed CD23 and CD163, respectively. Microarray analysis was used to evaluate the alteration of gene expression of lung cancer cell line (A549) after coculture with different subtype TAMs (M0, M1, M2a and M2c). Bioinformatics analysis indicated that TAMs mainly alter cell cycle, coagulation and cell adhesion pathways in tumor cells. The M0, M2a and M2c promoted the invasion ability of A549 after coculture for 2days. Otherwise, M1 condition medium reduced cell viability and induced apoptosis of A549 but not H1299, the p53 null cell line. Moreover, the M2 macrophage condition medium but not M1 increased the drug resistance ability of A549 cells after long-term coculture. More importantly, in vivo tumorigenesis assay indicated that M2 can promote tumor growth and angiogenesis. Different subtypes of macrophages can modify behaviors of cancer cells and may contribute to tumor progression. Further studies in the detailed mechanisms involved in the interaction of TAMs and cancer cells will unveil the role of macrophages in tumor progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1577. doi:10.1158/1538-7445.AM2011-1577
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