Absorption of flavones in Gegenqinlian decoction and different compatibilities in rat everted gut scas

Abstract

Using rat everted gut sacs to investigate the intestinal absorption characteristics of flavones in Gegenqinlian decoction and different compatibilities. In the research, the intestinal absorption mechanism of the active ingredients including puerarin, daidzin, liquiritin, scutellarin, baicalin and wogonoside in Gegenqinlian decoction were studied; and then the influences of different intestinal segments, different drug concentrations on the absorption of ingredients were discussed; finally, the absorption characteristics of the active compounds in different compatibility were also be compared. The absorption mode of the six compounds we disscussed was linearity, R2 > 0.9, which was consistent with the zero order rate process. The mechanism of absorption of puerarin, daidzin, liquiritin and scutellarin across intestinal sacs was passive diffusion, while active transportation was also existed. The Ka of baicalin and wogonoside increased along with the raising dosage, which indicated the passive absorption of the two ingredients. Different parts of the intestinal absorption experiments of Gegenqinlian decoction showed that the best absorption site of puerarin, daidzin and scutellarin was jejunum; liquiritin and baicalin can be absorbed better in colon; and the best absorption part of wogonoside was ileum. In the section of comparing the Ka of different compatibilities, it was founded that the absorption of puerarin, daidzin, liquiritin, baicalin in Gegenqinlian decoction were best; in the four intestinal segments, scutellarin and wogonoside were also absorbed best in Gegenqinlian decoction except for the duodenum where the best compatibility was Gegenqinlian group. For ingerdients, there was selective absorption in intestinal sacs. The intestinal absorption characteristics of each component in different compatibilities were different, however, the Gegenqinlian decoction was the optimal.