Abstract
Green-spore revertants of a dominant-yellow conidial-color mutant ( yB35) of Aspergillus nidulans were found to be abnormal in sexual development. Sexual fruiting bodies (cleistothecia) either were completely absent or were overproduced. For each of three revertants analyzed, reversion was due to a second-site suppressor mutation unlinked to the yB locus. Outcrossed strains that carried the suppressor mutations free of the yB mutation retained the aberrations in sexual development exhibited by the corresponding revertants. This finding provides a powerful screening method for rare sexual cycle mutants, since the yB revertants are manifested as easily identified green patches on a lawn of yellow conidia. Possible mechanisms of suppression of the yB phenotype are discussed.
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