Abstract
Objectives: To assess circulating plasma DNA (pDNA) as a possible predictive factor for pregnancy associated metabolic complications. Methods: Blood samples were collected from 200 subjects, in the second trimester of pregnancy from Obstetrics and Gynecology Clinic, KGMU, Lucknow, India. pDNA from all samples was quantified spectrophotometrically and in real-time quantitative PCR (qPCR), using β-actin and GAPDH housekeeping gene primers. Statistics was applied using SPSS (ver. 21.0). Results: The qPCR analysis for β-actin and GAPDH at II trimester of pregnancy demonstrated a significant increase (P = 0.001) in concentration of pDNA in pre-eclamptic cases as compared with normal healthy pregnant subjects. Similarly, significant increase (P = 0.003 and 0.002) was observed in gestational diabetes (GDM) cases for β-actin and GAPDH gene primers respectively. Conclusion: Our results confirmed that concentrations of circulating plasma DNA may be used as a possible early predictive marker for pregnancy associated metabolic complications.
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