Abstract
The precise detection of endogenous biothiols such as Glutathione (GSH), Cysteine (Cys) and Homocysteine (HCy) is a long-standing human health concern. A dual-channel fluorescent probe (Cy-DC) composed of two fluorescence reporting units (dicyanomethylene-4H-pyran and cyanine) with ether linker for distinguishing different endogenous biothiols was designed and synthesized. Due to the two well-resolved emission bands, this probe possesses an outstanding capability for selectively sensing of endogenous GSH spatiotemporally and synchronously. Nevertheless, in the near infrared (NIR) channel (λex = 700 nm, λem = 810 nm), the probe can produce strong fluorescence only when it responds to GSH. Besides, the cells (L02 and U87) imaging, it also demonstrated that Cy-DC could successfully discriminate between GSH and Cys/Hcy with high sensitivity with the limit of detection (LOD) is 24 nM and 32 nM (3*SD/K). Particularly, the probe was applied in detecting solid tumor by the naked-eye and NIR imaging successfully, which revealed that the probe has promising prospects in clinical diagnosis applications.
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