Abstract
A new monoclonal antibody (MAb) specific to plasma fibronectin, named MO, which was produced by immunizing a mouse with fragments of fibronectin isolated from human plasma, was found to bind to the surface of a subpopulation of peripheral blood polymorphonuclear neutrophils (PMNs) in the presence of physiological concentration of soluble fibronectin. MAb MO had no reactivity with other blood cells such as lymphocytes, monocytes, or platelets. The percentage of the MAb MO-reactive subpopulation of PMNs from healthy donors varied from 0 to 60%. Ten previously established MAbs against fibronectin tested in this study together with MAb MO had no reactivity with the surface of PMNs at all, when tested simultaneously with MAb MO. These results indicate that MAb MO can be a unique and useful tool for analyzing the structure and function of fibronectin-especially the fibronectin expressed on the surface of PMNs.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.