Abstract
Cytoplasmic droplets (CD) associated with mammalian sperm have traditionally been investigated after isolation from ejaculated sperm cells, or in fixed tissue sections from the epididymides. Many of the current techniques for preparing spermatozoa for immunofluorescence assay (IFA) induce separation of distal cytoplasmic droplets, particularly when membrane permeabilization is required. This article describes a technique capable of maintaining distal cytoplasmic droplets in situ on ejaculated porcine spermatozoa throughout the process of permeabilization and immunostaining. Key steps in this technique include fixation with 0.2% glutaraldehyde, permeabilization with 0.05% TX-100, and microtube incubations for IFA. Utilizing glutaraldehyde fixation, this technique yielded a mean retention rate of 88% for distal cytoplasmic droplets on ejaculated porcine spermatozoa.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
