Abstract
SUMMARY—Sporulation of the two variants, rough and smooth, of B. stearothermophilus NCA 1518 was studied in three complex media, nutrient agar, nutrient broth and trypticase soy agar. The rough variant sporulated best on nutrient agar enriched with one ppm manganese with or without yeast extract but did not sporulate in liquid media. Aerated broth fortified with yeast extract and one ppm manganese was found to be the best sporulating medium for the smooth variant. The effects of calcium, cobalt, and dextrose on the sporulation of the two variants are discussed.
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