A STUDY ON CLINICO-MICROBIOLOGICAL PROFILE OF CONJUNCTIVAL FLORA IN CONGENITAL DACROCYSTITIS

Abstract

Dacryocystitis is an inammation of the lacrimal sac and duct. It may be congenital or acquired.It is an important cause of ocular morbidity both in children and adults. To isolate & identify the causati PURPOSE- ve bacteria from clinically diagnosed cases of congenital dacryocystitis & to study the organism in relation to clinical prole. -Gram฀s stain MATERIALS & METHODS & Giemsa stain ,Culture media,10 % KOH preparation , MacConkey฀s agar, 5% Sheep Blood agar, Chocolate agar .Biochemical tests on Isolated colonies from the plates were identied by Colony morphology, Gram฀s stain, Oxidase test, Catalase test, and Motility.For further identication isolated colony was inoculated into peptone water and Hiss serum for fastidious bacteria, which was incubated aerobically and inoculated into appropriate medias for biochemical tests like Indole production, Methyl red, Voges-proskauer test, Citrate utilization, Urease hydrolysis, Sugar fermentation, Triple Sugar Iron agar, Nitrate reduction test and Amino acids decarboxylase tests. The specic tests like Inulin fermentation, Optochin sensitivity test and Bile solubility test were done. Antimicrobial susceptibility tests: The standardized Kirby-Bauer disc diffusion test of the Clinical and Laboratory Standards institute (formerly NCCLS) was used for testing. The media used was Mueller-Hinton agar for non-fastidious organisms. 5% Sheep Blood agar added to Mueller-Hinton agar for fastidious organisms and Haemophilus Test Medium was used for testing Haemophilus inuenzae and it was read after 16-18 hours. The zone of inhibition was measured and reported as Susceptible or Resistant. For detection of methicillin resistant Staphylococcus aureus detection disc tests for methicillin resistance was done using 10μg oxacillin disc on Mueller-Hinton agar containing 4% sodium chloride. In our study , femal RESULTS- es are more (7) as compared to males (4)& the culture positive cases were 10 and in 1 case culture showed no bacterial growth. The most frequent Gram-positive isolates are Streptococcus pneumoniae 7 (63.63%) followed by Staphylococcus aureus 1 (9.09%) and the most frequent Gram-negative isolates are Pseudomonas aeruginosa 3 (27.27%). In our study the Gram-positive isolates were most sensitive to CONCLUSION- Vancomycin (100%), followed by Tobramycin and Linezolid (97.14 %). Least sensitive antibiotic against Gram-positive organisms was Penicillin (51.42 %). The Gram-negative organisms were most sensitive to Tobramycin and Gentamicin (100%), followed by Amikacin, Oaxacin (92.59 %), Cefepime (88.88 %). Least sensitive antibiotic against Gram-negative organisms was Ciprooxacin (77.77 %)