Abstract
The present study was designed to simultaneously isolate the less polar ginsenosides from the flower buds of Panax ginseng (FBPG). Five ginsenosides, including a pair of new 20-methoxyl isomers, were extracted from FBPG and purified through a five-step integrated strategy, by combining ultrasonic extraction, Diaion Hp-20 macroporous resin column enrichment, solid phase extraction (SPE), reversed-phase high-performance liquid chromatography (RP-HPLC) analysis and preparation, and nuclear magnetic resonance (NMR) analysis. The quantification of the five ginsenosides was also discussed by a developed method with validations within acceptable limits. Ginsenoside Rg5 showed content of about 1% in FBPG. The results indicated that FBPG might have many different ginsenosides with diverse chemical structures, and the less polar ginsenosides were also important to the quality control and standardization of FBPG.
Highlights
To obtain the high yield of less polar ginsenoside from flower buds of Panax ginseng (FBPG) extracts, Diaion HP-20 macroporous resin was used to remove the polar ginsenosides by the elution with H2 O
The last fraction was rich in less polar ginsenosides (Figure S1), which were used to further isolate and purify specific ginsenosides
The purified ginsenosides included a pair of new 20-methoxyl isomers and three known compounds which were identified for the first time from FBPG
Summary
More than 100 naturally-occurring ginsenosides have been isolated from roots, leaves/stems, fruits, and/or flower buds of PG [5,6,7,8]. In addition to PG root, such as leaves and stems, flower buds and fruits were studied for their medicinal values [13,14,15]. The ginsenosides in the flower buds of Panax ginseng (FBPG) gradually attracted people’s attention, and it was considered as a valuable functional food with medicinal potential. People commonly use it as tea for anti-fatigue and for enhancing immunity [16]
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